DNA

Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo

Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo

Grownup skeletal muscle tissue harbors a stem cell inhabitants that’s indispensable for its means to regenerate. Upon muscle harm, muscle stem cells go away their quiescent state and activate the myogenic program in the end resulting in the restore of broken tissue concomitant with the replenishment of the muscle stem cell pool. Numerous elements affect …

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Co-culture with Tetragenococcus halophilus improved the ethanol tolerance of Zygosaccharomyces rouxii by maintaining cell surface properties

Co-culture with Tetragenococcus halophilus improved the ethanol tolerance of Zygosaccharomyces rouxii by maintaining cell surface properties

The buildup of ethanol has a damaging impact on the viability and fermentation efficiency of microorganisms in the course of the manufacturing of fermented meals due to its toxicity. On this research, we investigated the impact of co-culture with Tetragenococcus halophilus on ethanol stress resistance of Zygosaccharomyces rouxii. The outcome confirmed that co-culture with T. …

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Co-culture of Human Stem Cell Derived Neurons and Oligodendrocyte Progenitor Cells

Co-culture of Human Stem Cell Derived Neurons and Oligodendrocyte Progenitor Cells

Crosstalk between neurons and oligodendrocytes is necessary for correct mind functioning. A number of co-culture strategies have been developed to check oligodendrocyte maturation, myelination or the impact of oligodendrocytes on neurons. Nevertheless, most of those strategies include cells derived from animal fashions. Within the present protocol, we co-culture human neurons with human oligodendrocytes. Neurons and …

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Quantifying HIV-1-Mediated Gut CD4 + T Cell Deathin the Lamina Propria Aggregate Culture (LPAC) Model

Quantifying HIV-1-Mediated Gut CD4 + T Cell Deathin the Lamina Propria Aggregate Culture (LPAC) Model

Intestine CD4 T cells are main targets of HIV-1 and are massively depleted early throughout an infection. To higher perceive the mechanisms governing HIV-1-mediated CD4 T cell loss of life, we developed the physiologically-relevant Lamina Propria Combination Tradition (LPAC) mannequin. The LPAC mannequin is good for learning CD4 T cell loss of life induced by …

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