Long filopodia and tunneling nanotubes define new phenotypes of breast cancer cells in 3D cultures

Long filopodia and tunneling nanotubes define new phenotypes of breast cancer cells in 3D cultures

Most cancers cell invasion into the encircling extracellular matrix (ECM) takes place when cell-cell junctions are disrupted upon epithelial-to-mesenchymal transition (EMT). Each most cancers cell-stroma and cell-cell crosstalk are important to help the continual tumor invasion. Most cancers cells launch microvesicles and exosomes containing bioactive molecules and sign peptides, that are recruited by neighboring cells …

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Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo

Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo

Grownup skeletal muscle tissue harbors a stem cell inhabitants that’s indispensable for its means to regenerate. Upon muscle harm, muscle stem cells go away their quiescent state and activate the myogenic program in the end resulting in the restore of broken tissue concomitant with the replenishment of the muscle stem cell pool. Numerous elements affect …

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Co-culture with Tetragenococcus halophilus improved the ethanol tolerance of Zygosaccharomyces rouxii by maintaining cell surface properties

Co-culture with Tetragenococcus halophilus improved the ethanol tolerance of Zygosaccharomyces rouxii by maintaining cell surface properties

The buildup of ethanol has a damaging impact on the viability and fermentation efficiency of microorganisms in the course of the manufacturing of fermented meals due to its toxicity. On this research, we investigated the impact of co-culture with Tetragenococcus halophilus on ethanol stress resistance of Zygosaccharomyces rouxii. The outcome confirmed that co-culture with T. …

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Co-culture of Human Stem Cell Derived Neurons and Oligodendrocyte Progenitor Cells

Co-culture of Human Stem Cell Derived Neurons and Oligodendrocyte Progenitor Cells

Crosstalk between neurons and oligodendrocytes is necessary for correct mind functioning. A number of co-culture strategies have been developed to check oligodendrocyte maturation, myelination or the impact of oligodendrocytes on neurons. Nevertheless, most of those strategies include cells derived from animal fashions. Within the present protocol, we co-culture human neurons with human oligodendrocytes. Neurons and …

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Quantifying HIV-1-Mediated Gut CD4 + T Cell Deathin the Lamina Propria Aggregate Culture (LPAC) Model

Quantifying HIV-1-Mediated Gut CD4 + T Cell Deathin the Lamina Propria Aggregate Culture (LPAC) Model

Intestine CD4 T cells are main targets of HIV-1 and are massively depleted early throughout an infection. To higher perceive the mechanisms governing HIV-1-mediated CD4 T cell loss of life, we developed the physiologically-relevant Lamina Propria Combination Tradition (LPAC) mannequin. The LPAC mannequin is good for learning CD4 T cell loss of life induced by …

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Production of Recombinant Adeno-associated Virus Vectors Using Suspension HEK293 Cells and Continuous Harvest of Vector From the Culture Media for GMP FIX and FLT1 Clinical Vector.

Production of Recombinant Adeno-associated Virus Vectors Using Suspension HEK293 Cells and Continuous Harvest of Vector From the Culture Media for GMP FIX and FLT1 Clinical Vector.

Adeno-associated virus (AAV) has proven nice promise as a gene remedy vector in a number of points of preclinical and medical purposes. Many developments together with new serotypes in addition to self-complementary vectors at the moment are coming into the clinic. With these ongoing vector developments, continued effort has been centered on scalable manufacturing processes …

Production of Recombinant Adeno-associated Virus Vectors Using Suspension HEK293 Cells and Continuous Harvest of Vector From the Culture Media for GMP FIX and FLT1 Clinical Vector. Read More »

Laminin enhances the growth of human neural stem cells in defined culture media.

Laminin enhances the growth of human neural stem cells in defined culture media.

BACKGROUND Human neural stem cells (hNSC) have the potential to offer novel cell-based therapies for neurodegenerative circumstances equivalent to a number of sclerosis and Parkinson’s illness. To be able to realise this purpose, protocols have to be developed that enable for giant portions of hNSC to be cultured effectively. As such, you will need to …

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Comparison of nanotube-protein corona composition in cell culture media.

Comparison of nanotube-protein corona composition in cell culture media.

In organic environments, nanomaterials affiliate with proteins forming a protein corona (PC). The PC might alter the nanomaterial’s pharmacokinetics and pharmacodynamics, thereby influencing toxicity. Utilizing a label-free mass spectrometry-based proteomics method, the composition of the PC is examined for a set of nanotubes (NTs) together with unmodified and carboxylated single- (SWCNT) and multi-walled carbon nanotubes …

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