Quantifying HIV-1-Mediated Gut CD4 + T Cell Deathin the Lamina Propria Aggregate Culture (LPAC) Model

Quantifying HIV-1-Mediated Gut CD4 + T Cell Deathin the Lamina Propria Aggregate Culture (LPAC) Model

Intestine CD4 T cells are main targets of HIV-1 and are massively depleted early throughout an infection. To higher perceive the mechanisms governing HIV-1-mediated CD4 T cell loss of life, we developed the physiologically-relevant Lamina Propria Combination Tradition (LPAC) mannequin. The LPAC mannequin is good for learning CD4 T cell loss of life induced by clinically-relevant Transmitted/Founder (TF) HIV-1 strains and can also be appropriate for learning how enteric microbes and soluble elements (e.g., Kind I Interferons) affect LP CD4 T cell loss of life and performance. Right here, we element the protocol to ascertain LP CD4 T cell an infection utilizing a means of spinoculation, the following analysis of an infection ranges utilizing multicolor circulate cytometry and the dedication of general LP CD4 T cell loss of life utilizing absolute LP CD4 T cell counts. We additionally describe the preparation of virus shares of Transmitted/Founder (TF) HIV-1 infectious molecular clones that had been efficiently used within the LPAC mannequin.

Co-culture programs using reconstituted or artificial extracellular matrix (ECM) and micropatterning strategies have enabled the reconstruction of floor epithelial tissues. This method has been utilized within the regeneration, illness modeling and drug screening of the floor epithelia, such because the pores and skin and esophagus. However, the reconstruction of glandular epithelia would require extra intricate ECM organizations. Right here we describe a protocol for a novel three-dimensional organotypic co-culture system for the reconstruction of mammary glands that makes use of the discontinuous ECM. On this method, main mammary fibroblasts first set up a layer of the connective tissue wealthy in collagen I. Then, mammary epithelial cells kind acinar constructions, the useful glandular models, inside the laminin-rich basement membrane embedded within the connective tissue. This methodology permits for the regeneration of the in vivo-like structure of mammary glands and might be utilized for monitoring the real-time response of mammary glands to drug therapy.

Utilizing Imaging Move Cytometry to Characterize Extracellular Vesicles Remoted from Cell Tradition Media, Plasma or Urine

 

The flexibility to non-invasively detect particular injury to the kidney has been restricted. Identification of extracellular vesicles launched by cells, particularly when below duress, may permit for monitoring and identification of particular cell sorts inside the kidney which are harassed. We’ve got tailored a beforehand revealed conventional circulate cytometry methodology to be used with an imaging circulate cytometer (Amnis FlowSight) for figuring out EV launched by particular cell sorts and excreted into the urine or blood utilizing markers attribute of specific cells within the kidney. Right here we current a protocol using the Amnis FlowSight Imaging Move Cytometer to establish and quantify EV from the urine of sufferers with important hypertension and renovascular illness. Notably, EV remoted from cell tradition media and plasma may also be analyzed equally.

Dissolved oxygen and its availability to cells in tradition is an ignored variable which may have vital penalties on experimental analysis outcomes, together with reproducibility. Oxygen sensing pathways play key roles in cell progress and conduct and pericellular oxygen ranges ought to be managed when establishing in vitro fashions. Normal cell tradition strategies wouldn’t have ample management over pericellular oxygen ranges. Gradual diffusion by tradition media limits the precision of oxygen supply to cells, making it tough to precisely reproduce in vivo-like oxygen situations. Moreover, several types of cells devour oxygen at various charges and this may be affected by the density of rising cells. Right here, we describe a novel in vitro system that makes use of hypoxic chambers and oxygen-permeable tradition dishes to regulate pericellular oxygen ranges and supply speedy oxygen supply to adherent cells. This process is especially related for protocols learning results of speedy oxygen adjustments or intermittent hypoxia on mobile conduct. The system is cheap and simply assembled with out extremely specialised gear.

Isolation and Tradition of Single Myofiber and Immunostaining of Satellite tv for pc Cells from Grownup C57BL/6J Mice

 

Myofiber isolation adopted with ex vivo tradition may recapitulate and visualize satellite tv for pc cells (SCs) activation, proliferation, and differentiation. This strategy might be taken to know the physiology of satellite tv for pc cells and the molecular mechanism of regulatory elements, by way of the involvement of intrinsic elements over SCs quiescence, activation, proliferation and differentiation. Single myofiber tradition has a number of benefits that the standard strategy resembling FASC and cryosection couldn’t compete with. For instance, myofiber isolation and tradition might be used to watch SCs activation, proliferation and differentiation at a steady method inside their physiological “area of interest” setting whereas FACS or cryosection may solely seize single time-point upon exterior stimulation to activate satellite tv for pc cells by BaCl2, Cardiotoxin or ischemia.

Whole Blood RNA Extraction Kit

K1012050 1 Kit
EUR 333
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Rabbit Whole Blood (Sterile Sodium Citrate)

20-abx800010
  • EUR 578.00
  • EUR 411.00
  • 100 ml
  • 50 ml
  • Shipped within 1-5 working days.

Rabbit Whole Blood (Sterile Sodium EDTA)

20-abx800012
  • EUR 578.00
  • EUR 411.00
  • 100 ml
  • 50 ml
  • Shipped within 1-5 working days.

Rabbit Whole Blood (Sterile Sodium Heparin)

20-abx800014
  • EUR 578.00
  • EUR 411.00
  • 100 ml
  • 50 ml
  • Shipped within 1-5 working days.

Sheep Whole Blood (Sterile Sodium Citrate)

20-abx800027
  • EUR 314.00
  • EUR 272.00
  • 100 ml
  • 50 ml
  • Shipped within 1-5 working days.

Sheep Whole Blood (Sterile Sodium EDTA)

20-abx800029
  • EUR 314.00
  • EUR 272.00
  • 100 ml
  • 50 ml
  • Shipped within 1-5 working days.

Sheep Whole Blood (Sterile Sodium Heparin)

20-abx800031
  • EUR 314.00
  • EUR 272.00
  • 100 ml
  • 50 ml
  • Shipped within 1-5 working days.

Goat Whole Blood (Sterile Sodium Citrate)

20-abx800042
  • EUR 314.00
  • EUR 272.00
  • 100 ml
  • 50 ml
  • Shipped within 1-5 working days.

Goat Whole Blood (Sterile Sodium EDTA)

20-abx800044
  • EUR 314.00
  • EUR 272.00
  • 100 ml
  • 50 ml
  • Shipped within 1-5 working days.

Goat Whole Blood (Sterile Sodium Heparin)

20-abx800046
  • EUR 314.00
  • EUR 272.00
  • 100 ml
  • 50 ml
  • Shipped within 1-5 working days.

Horse Whole Blood (Sterile Sodium Citrate)

20-abx800057
  • EUR 425.00
  • EUR 578.00
  • EUR 356.00
  • 100 ml
  • 500 ml
  • 50 ml
  • Shipped within 1-5 working days.

Horse Whole Blood (Sterile Sodium Citrate)

abx800057-1L 1 L
EUR 690
  • Shipped within 1-5 working days.

Horse Whole Blood (Sterile Sodium EDTA)

20-abx800059
  • EUR 425.00
  • EUR 578.00
  • EUR 356.00
  • 100 ml
  • 500 ml
  • 50 ml
  • Shipped within 1-5 working days.

Horse Whole Blood (Sterile Sodium EDTA)

abx800059-1L 1 L
EUR 690
  • Shipped within 1-5 working days.

Horse Whole Blood (Sterile Sodium Heparin)

20-abx800061
  • EUR 425.00
  • EUR 578.00
  • EUR 356.00
  • 100 ml
  • 500 ml
  • 50 ml
  • Shipped within 1-5 working days.

Horse Whole Blood (Sterile Sodium Heparin)

abx800061-1L 1 L
EUR 690
  • Shipped within 1-5 working days.

Donkey Whole Blood (Sterile Sodium Citrate)

20-abx800072
  • EUR 648.00
  • EUR 913.00
  • EUR 467.00
  • 100 ml
  • 500 ml
  • 50 ml
  • Shipped within 1-5 working days.

Donkey Whole Blood (Sterile Sodium Citrate)

abx800072-1L 1 L
EUR 1094
  • Shipped within 1-5 working days.

Donkey Whole Blood (Sterile Sodium EDTA)

20-abx800074
  • EUR 648.00
  • EUR 913.00
  • EUR 467.00
  • 100 ml
  • 500 ml
  • 50 ml
  • Shipped within 1-5 working days.

Donkey Whole Blood (Sterile Sodium EDTA)

abx800074-1L 1 L
EUR 1094
  • Shipped within 1-5 working days.

Donkey Whole Blood (Sterile Sodium Heparin)

20-abx800076
  • EUR 648.00
  • EUR 913.00
  • EUR 467.00
  • 100 ml
  • 500 ml
  • 50 ml
  • Shipped within 1-5 working days.

Donkey Whole Blood (Sterile Sodium Heparin)

abx800076-1L 1 L
EUR 1094
  • Shipped within 1-5 working days.

Camel Whole Blood (Sterile Sodium Citrate)

20-abx800087
  • EUR 718.00
  • EUR 1748.00
  • EUR 537.00
  • 100 ml
  • 500 ml
  • 50 ml
  • Shipped within 1-5 working days.

Camel Whole Blood (Sterile Sodium Citrate)

abx800087-1L 1 L
EUR 2736
  • Shipped within 1-5 working days.

Camel Whole Blood (Sterile Sodium EDTA)

20-abx800089
  • EUR 718.00
  • EUR 1748.00
  • EUR 537.00
  • 100 ml
  • 500 ml
  • 50 ml
  • Shipped within 1-5 working days.

Camel Whole Blood (Sterile Sodium EDTA)

abx800089-1L 1 L
EUR 2736
  • Shipped within 1-5 working days.

Camel Whole Blood (Sterile Sodium Heparin)

20-abx800091
  • EUR 718.00
  • EUR 1748.00
  • EUR 537.00
  • 100 ml
  • 500 ml
  • 50 ml
  • Shipped within 1-5 working days.

Camel Whole Blood (Sterile Sodium Heparin)

abx800091-1L 1 L
EUR 2736
  • Shipped within 1-5 working days.

Dog Whole Blood (Sterile Sodium Citrate)

20-abx800117
  • EUR 620.00
  • EUR 439.00
  • 100 ml
  • 50 ml
  • Shipped within 1-5 working days.

Dog Whole Blood (Sterile Sodium EDTA)

20-abx800119
  • EUR 620.00
  • EUR 439.00
  • 100 ml
  • 50 ml
  • Shipped within 1-5 working days.

Dog Whole Blood (Sterile Sodium Heparin)

20-abx800121
  • EUR 620.00
  • EUR 439.00
  • 100 ml
  • 50 ml
  • Shipped within 1-5 working days.

Chicken Whole Blood (Sterile Sodium Citrate)

20-abx800132
  • EUR 398.00
  • EUR 300.00
  • 100 ml
  • 50 ml
  • Shipped within 1-5 working days.

Chicken Whole Blood (Sterile Sodium EDTA)

20-abx800134
  • EUR 398.00
  • EUR 300.00
  • 100 ml
  • 50 ml
  • Shipped within 1-5 working days.

Chicken Whole Blood (Sterile Sodium Heparin)

20-abx800136
  • EUR 398.00
  • EUR 300.00
  • 100 ml
  • 50 ml
  • Shipped within 1-5 working days.

Human Whole Blood Monocyte Isolation Kit

K933-30
EUR 376

Zenoquick Whole Blood Total RNA Kit

Z5010-050 50 preps
EUR 482

Zenoquick Whole Blood Total RNA Kit

Z5010-100 2x50 preps
EUR 806

Rabbit Whole Blood (Non-Sterile Sodium EDTA)

20-abx800011
  • EUR 578.00
  • EUR 411.00
  • 100 ml
  • 50 ml
  • Shipped within 1-5 working days.

Rabbit Whole Blood (Non-Sterile Sodium Heparin)

20-abx800013
  • EUR 578.00
  • EUR 411.00
  • 100 ml
  • 50 ml
  • Shipped within 1-5 working days.

Sheep Whole Blood (Non-Sterile Sodium Citrate)

20-abx800026
  • EUR 314.00
  • EUR 272.00
  • 100 ml
  • 50 ml
  • Shipped within 1-5 working days.

Sheep Whole Blood (Non-Sterile Sodium EDTA)

20-abx800028
  • EUR 314.00
  • EUR 272.00
  • 100 ml
  • 50 ml
  • Shipped within 1-5 working days.

Sheep Whole Blood (Non-Sterile Sodium Heparin)

20-abx800030
  • EUR 314.00
  • EUR 272.00
  • 100 ml
  • 50 ml
  • Shipped within 1-5 working days.

Goat Whole Blood (Non-Sterile Sodium Citrate)

20-abx800041
  • EUR 314.00
  • EUR 272.00
  • 100 ml
  • 50 ml
  • Shipped within 1-5 working days.

Goat Whole Blood (Non-Sterile Sodium EDTA)

20-abx800043
  • EUR 314.00
  • EUR 272.00
  • 100 ml
  • 50 ml
  • Shipped within 1-5 working days.

Goat Whole Blood (Non-Sterile Sodium Heparin)

20-abx800045
  • EUR 314.00
  • EUR 272.00
  • 100 ml
  • 50 ml
  • Shipped within 1-5 working days.

Horse Whole Blood (Non-Sterile Sodium Citrate)

20-abx800056
  • EUR 384.00
  • EUR 509.00
  • EUR 314.00
  • 100 ml
  • 500 ml
  • 50 ml
  • Shipped within 1-5 working days.

Horse Whole Blood (Non-Sterile Sodium Citrate)

abx800056-1L 1 L
EUR 592
  • Shipped within 1-5 working days.

Horse Whole Blood (Non-Sterile Sodium EDTA)

20-abx800058
  • EUR 384.00
  • EUR 509.00
  • EUR 314.00
  • 100 ml
  • 500 ml
  • 50 ml
  • Shipped within 1-5 working days.

Horse Whole Blood (Non-Sterile Sodium EDTA)

abx800058-1L 1 L
EUR 592
  • Shipped within 1-5 working days.

Horse Whole Blood (Non-Sterile Sodium Heparin)

20-abx800060
  • EUR 384.00
  • EUR 509.00
  • EUR 314.00
  • 100 ml
  • 500 ml
  • 50 ml
  • Shipped within 1-5 working days.

Horse Whole Blood (Non-Sterile Sodium Heparin)

abx800060-1L 1 L
EUR 592
  • Shipped within 1-5 working days.

Donkey Whole Blood (Non-Sterile Sodium Citrate)

20-abx800071
  • EUR 565.00
  • EUR 787.00
  • EUR 411.00
  • 100 ml
  • 500 ml
  • 50 ml
  • Shipped within 1-5 working days.

Donkey Whole Blood (Non-Sterile Sodium Citrate)

abx800071-1L 1 L
EUR 940
  • Shipped within 1-5 working days.

Donkey Whole Blood (Non-Sterile Sodium EDTA)

20-abx800073
  • EUR 565.00
  • EUR 787.00
  • EUR 411.00
  • 100 ml
  • 500 ml
  • 50 ml
  • Shipped within 1-5 working days.

Donkey Whole Blood (Non-Sterile Sodium EDTA)

abx800073-1L 1 L
EUR 940
  • Shipped within 1-5 working days.

Donkey Whole Blood (Non-Sterile Sodium Heparin)

20-abx800075
  • EUR 565.00
  • EUR 787.00
  • EUR 411.00
  • 100 ml
  • 500 ml
  • 50 ml
  • Shipped within 1-5 working days.

Donkey Whole Blood (Non-Sterile Sodium Heparin)

abx800075-1L 1 L
EUR 940
  • Shipped within 1-5 working days.

Camel Whole Blood (Non-Sterile Sodium Citrate)

20-abx800086
  • EUR 620.00
  • EUR 1483.00
  • EUR 467.00
  • 100 ml
  • 500 ml
  • 50 ml
  • Shipped within 1-5 working days.

Camel Whole Blood (Non-Sterile Sodium Citrate)

abx800086-1L 1 L
EUR 2305
  • Shipped within 1-5 working days.

Camel Whole Blood (Non-Sterile Sodium EDTA)

20-abx800088
  • EUR 620.00
  • EUR 1483.00
  • EUR 467.00
  • 100 ml
  • 500 ml
  • 50 ml
  • Shipped within 1-5 working days.

Camel Whole Blood (Non-Sterile Sodium EDTA)

abx800088-1L 1 L
EUR 2305
  • Shipped within 1-5 working days.

Camel Whole Blood (Non-Sterile Sodium Heparin)

20-abx800090
  • EUR 620.00
  • EUR 1483.00
  • EUR 467.00
  • 100 ml
  • 500 ml
  • 50 ml
  • Shipped within 1-5 working days.

Camel Whole Blood (Non-Sterile Sodium Heparin)

abx800090-1L 1 L
EUR 2305
  • Shipped within 1-5 working days.

Beagle dog Whole Blood (Sterile Sodium Citrate)

20-abx800102
  • EUR 857.00
  • EUR 578.00
  • 100 ml
  • 50 ml
  • Shipped within 1-5 working days.

Beagle dog Whole Blood (Sterile Sodium EDTA)

20-abx800104
  • EUR 857.00
  • EUR 578.00
  • 100 ml
  • 50 ml
  • Shipped within 1-5 working days.

Beagle dog Whole Blood (Sterile Sodium Heparin)

20-abx800106
  • EUR 857.00
  • EUR 578.00
  • 100 ml
  • 50 ml
  • Shipped within 1-5 working days.

Dog Whole Blood (Non-Sterile Sodium Citrate)

20-abx800116
  • EUR 551.00
  • EUR 384.00
  • 100 ml
  • 50 ml
  • Shipped within 1-5 working days.

Dog Whole Blood (Non-Sterile Sodium EDTA)

20-abx800118
  • EUR 551.00
  • EUR 384.00
  • 100 ml
  • 50 ml
  • Shipped within 1-5 working days.

Dog Whole Blood (Non-Sterile Sodium Heparin)

20-abx800120
  • EUR 551.00
  • EUR 384.00
  • 100 ml
  • 50 ml
  • Shipped within 1-5 working days.

Chicken Whole Blood (Non-Sterile Sodium Citrate)

20-abx800131
  • EUR 356.00
  • EUR 286.00
  • 100 ml
  • 50 ml
  • Shipped within 1-5 working days.

Chicken Whole Blood (Non-Sterile Sodium EDTA)

20-abx800133
  • EUR 356.00
  • EUR 286.00
  • 100 ml
  • 50 ml
  • Shipped within 1-5 working days.

Chicken Whole Blood (Non-Sterile Sodium Heparin)

20-abx800135
  • EUR 356.00
  • EUR 286.00
  • 100 ml
  • 50 ml
  • Shipped within 1-5 working days.

Human Whole Blood Polymorphonuclear Cell Isolation Kit

K483-30
EUR 376

Beagle dog Whole Blood (Non-Sterile Sodium Citrate)

20-abx800101
  • EUR 746.00
  • EUR 509.00
  • 100 ml
  • 50 ml
  • Shipped within 1-5 working days.

Beagle dog Whole Blood (Non-Sterile Sodium EDTA)

20-abx800103
  • EUR 746.00
  • EUR 509.00
  • 100 ml
  • 50 ml
  • Shipped within 1-5 working days.

Beagle dog Whole Blood (Non-Sterile Sodium Heparin)

20-abx800105
  • EUR 746.00
  • EUR 509.00
  • 100 ml
  • 50 ml
  • Shipped within 1-5 working days.

Human Healthy Donor K2 EDTA Whole Blood Plasma, Fresh

FP-005-10 NULL
EUR 0

Human Healthy Donor K2 EDTA Whole Blood Plasma, Fresh

FP-005-100 NULL
EUR 0

Human Healthy Donor K2 EDTA Whole Blood Plasma, Fresh

FP-005-200 NULL
EUR 0

Human Healthy Donor K2 EDTA Whole Blood Plasma, Fresh

FP-005-50 NULL
EUR 0

2019-nCoV IgG/IgM Rapid Test Cassette (Whole Blood/Serum/Plasma)

GEN-402-25tests 25 tests
EUR 244
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.

2019-nCoV IgG/IgM RapiCard InstaTest for Whole Blood/Serum/Plasma samples

GEN-176552-25tests 25 tests
EUR 276
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.

2019-nCoV IgG/IgM RapiCard InstaTest for Whole Blood/Serum/Plasma samples

GEN-176552-50tests 50 tests
EUR 472
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.

Histone (Whole) Antibody

abx023918-2ml 2 ml
EUR 509
  • Shipped within 5-10 working days.

Rat Whole Heart

PC35133 P0 Rat - Whole Heart X2
EUR 1341

Mouse Whole Heart

PC35135 P0 Mouse - Whole heart X2
EUR 1341

2019-nCoV IgG/IgM RapiCard InstaTest Single use kit for Fingerstick Whole Blood samples

GEN-176553-25tests 25 tests
EUR 275
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.

2019-nCoV IgG/IgM RapiCard InstaTest Single use kit for Fingerstick Whole Blood samples

GEN-176553-50tests 50 tests
EUR 470
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.

CMV protein (whole cell)

30-AC70 1 ml
EUR 403
Description: Purified native CMV protein (whole cell)

Monkey IgM (whole molecule)

31R-AI093 250 µg
EUR 417
Description: Monkey whole IgM

Whole Human Serum antibody

20-1727 1 L
EUR 30
Description: Goat polyclonal Whole Human Serum antibody

Whole Human Serum antibody

20-S1110G000-V0 10 ml
EUR 133
Description: Goat polyclonal Whole Human Serum antibody

Goat Anti-human Chorionic Gonadotropin Whole (hCG-Whole) IgG aff pure

HCG14-A 1 mg
EUR 286

Blood PCR Kit

20-abx09801320ulSystems
  • EUR 258.00
  • EUR 565.00
  • 100 rxns × 20 ul Systems
  • 500 rxns × 20 ul Systems
  • Shipped within 5-10 working days.

Blood RNA Kit

20-abx098092
  • EUR 105.00
  • EUR 342.00
  • 2 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

Arabidopsis Thaliana Whole Tissue Lysate

PABL-1300 50 ug
EUR 164

Tomato Plant Whole Tissue Lysate

PABL-1301 50 ug
EUR 164

Barley Plant Whole Tissue Lysate

PABL-1302 50 ug
EUR 164

Beet Plant Whole Tissue Lysate

PABL-1303 50 ug
EUR 164

Corn Plant Whole Tissue Lysate

PABL-1304 50 ug
EUR 164

Cotton Plant Whole Tissue Lysate

PABL-1305 50 ug
EUR 164

Oat Plant Whole Tissue Lysate

PABL-1307 50 ug
EUR 164

Spinach Plant Whole Tissue Lysate

PABL-1308 50 ug
EUR 164

Sunflower Plant Whole Tissue Lysate

PABL-1309 50 ug
EUR 164

Wheat Plant Whole Tissue Lysate

PABL-1310 50 ug
EUR 164

HeLa Cell Lysate (Whole Cell)

LYSATE0001 200ug
EUR 150
Description: This whole cell lysate is prepared from Hela cells using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human Hela Whole Cell Lysate

LYSATE0023 200ug
EUR 150
Description: This cell lysate is prepared from human hela using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human A549 Whole Cell Lysate

LYSATE0025 200ug
EUR 150
Description: This cell lysate is prepared from human A549 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human Colo320 Whole Cell Lysate

LYSATE0026 200ug
EUR 150
Description: This cell lysate is prepared from human Colo320 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human Jurkat Whole Cell Lysate

LYSATE0027 200ug
EUR 150
Description: This cell lysate is prepared from human Jurkat using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human SW620 Whole Cell Lysate

LYSATE0028 200ug
EUR 150
Description: This cell lysate is prepared from human SW620 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human HepG2 Whole Cell Lysate

LYSATE0029 200ug
EUR 150
Description: This cell lysate is prepared from human HepG2 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human K562 Whole Cell Lysate

LYSATE0031 200ug
EUR 150
Description: This cell lysate is prepared from human K562 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human 293T Whole Cell Lysate

LYSATE0032 200ug
EUR 150
Description: This cell lysate is prepared from human 293T using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human 22RV1 Whole Cell Lysate

LYSATE0033 200ug
EUR 150
Description: This cell lysate is prepared from human 22RV1 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Human U2OS Whole Cell Lysate

LYSATE0034 200ug
EUR 150
Description: This cell lysate is prepared from human U2OS using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Rat RH35 Whole Cell Lysate

LYSATE0036 200ug
EUR 150
Description: This cell lysate is prepared from rat RH35 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Rat PC12 Whole Cell Lysate

LYSATE0037 200ug
EUR 150
Description: This cell lysate is prepared from rat PC12 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

EpiQuik Whole Cell Extraction Kit

OP-0003 100 Extractions
EUR 270.4
Description: Ask the seller for details

Mouse Heart Whole tissue lysate

MAL-1401 1 mg
EUR 524

Mouse Lung Whole tissue lysate

MAL-1402 1 mg
EUR 524

Mouse Brain Whole tissue lysate

MAL-1403 1 mg
EUR 524

Mouse Liver Whole tissue lysate

MAL-1404 1 mg
EUR 524

Mouse Kidney Whole tissue lysate

MAL-1405 1 mg
EUR 524

Mouse Spleen Whole tissue lysate

MAL-1406 1 mg
EUR 524

Mouse Thymus Whole tissue lysate

MAL-1409 1 mg
EUR 524

Mouse Bladder Whole tissue lysate

MAL-1410 1 mg
EUR 773

Mouse Colon Whole tissue lysate

MAL-1411 1 mg
EUR 524

Mouse Cerebellum Whole tissue lysate

MAL-1412 1 mg
EUR 524

Mouse Cerebrum Whole tissue lysate

MAL-1413 100ug
EUR 213

Mouse Pancreas Whole tissue lysate

MAL-1414 1 mg
EUR 524

Mouse Stomach Whole tissue lysate

MAL-1415 1 mg
EUR 524

Mouse Testis Whole tissue lysate

MAL-1416 1 mg
EUR 524

Mouse Adrenal Whole tissue lysate

MAL-1417 100ug
EUR 213

Moreover, in vitro transfection with siRNA or overexpression vector might be carried out below ex vivo tradition to know the detailed molecular operate of a selected gene on SCs physiology. With these benefits, the physiological state of SCs might be analyzed at a number of designated time-points by immunofluorescence staining. On this protocol, we offer an environment friendly and sensible protocol to isolate single myofiber from EDL muscle, adopted with ex vivo tradition and immunostaining.