Comparison of nanotube-protein corona composition in cell culture media.

Co-culture of Human Stem Cell Derived Neurons and Oligodendrocyte Progenitor Cells

Co-culture of Human Stem Cell Derived Neurons and Oligodendrocyte Progenitor Cells

Crosstalk between neurons and oligodendrocytes is necessary for correct mind functioning. A number of co-culture strategies have been developed to check oligodendrocyte maturation, myelination or the impact of oligodendrocytes on neurons. Nevertheless, most of those strategies include cells derived from animal fashions. Within the present protocol, we co-culture human neurons with human oligodendrocytes. Neurons and …

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Quantifying HIV-1-Mediated Gut CD4 + T Cell Deathin the Lamina Propria Aggregate Culture (LPAC) Model

Quantifying HIV-1-Mediated Gut CD4 + T Cell Deathin the Lamina Propria Aggregate Culture (LPAC) Model

Intestine CD4 T cells are main targets of HIV-1 and are massively depleted early throughout an infection. To higher perceive the mechanisms governing HIV-1-mediated CD4 T cell loss of life, we developed the physiologically-relevant Lamina Propria Combination Tradition (LPAC) mannequin. The LPAC mannequin is good for learning CD4 T cell loss of life induced by …

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Comparison of nanotube-protein corona composition in cell culture media.

Comparison of nanotube-protein corona composition in cell culture media.

In organic environments, nanomaterials affiliate with proteins forming a protein corona (PC). The PC might alter the nanomaterial’s pharmacokinetics and pharmacodynamics, thereby influencing toxicity. Utilizing a label-free mass spectrometry-based proteomics method, the composition of the PC is examined for a set of nanotubes (NTs) together with unmodified and carboxylated single- (SWCNT) and multi-walled carbon nanotubes …

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