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Scalable Production of Equine Platelet Lysate for Multipotent Mesenchymal Stromal Cell Culture

Scalable Production of Equine Platelet Lysate for Multipotent Mesenchymal Stromal Cell Culture

Antibodies, Assay Kits, Blog, cDNA, Clia Kits, Comparison of nanotube-protein corona composition in cell culture media., Culture Cells, Devices, DNA, DNA Templates, DNA Testing, Elisa Kits, Enzymes, Exosomes, Gels, Laminin enhances the growth of human neural stem cells in defined culture media., Medium & Serums, NATtrol, Panel, Particles, PCR, Pcr Kits, Peptides, Production of Recombinant Adeno-associated Virus Vectors Using Suspension HEK293 Cells and Continuous Harvest of Vector From the Culture Media for GMP FIX and FLT1 Clinical Vector., Reagents, Recombinant Proteins, Ria Kits, RNA, Test Kits, Western Blot / By Isabella Frazier

Translation of multipotent mesenchymal stromal cell (MSC)-based therapies is advancing in human and veterinary medication. One important subject is the in vitro tradition of MSC earlier than medical use. Utilizing fetal bovine serum (FBS) as complement to the basal medium continues to be the gold customary for cultivation of many cell varieties together with equine MSC. Alternate options are being explored, with substantial success utilizing platelet lysate-supplemented media for human MSC. Nevertheless, progress lags behind within the veterinary area. The goal of this research was to determine a scalable protocol for equine platelet lysate (ePL) manufacturing and to check the ePL in equine MSC tradition. Complete blood was harvested into blood assortment baggage from 20 wholesome horses. After checking pattern supplies for pathogen contamination, samples from 19 animals have been included. Platelet concentrates have been ready utilizing a buffy coat methodology. Platelets, platelet-derived development issue BB, and reworking development issue β1 concentrations have been elevated within the concentrates in contrast with entire blood or serum (p < 0.05), whereas white blood cells have been decreased (p < 0.05).

The concentrates have been lysed utilizing freeze/thaw cycles, which eradicated the cells whereas development issue concentrations have been maintained. Donor age negatively correlated with platelet and development issue concentrations after processing (p < 0.05). Lastly, all lysates have been pooled and the ePL was evaluated as tradition medium complement compared with FBS, utilizing adipose-derived MSC from 4 unrelated donor horses. MSC proliferated properly in 10% FBS in addition to in 10% ePL. Nevertheless, utilizing 5 or 2.5% ePL entailed extremely inconsistent proliferation or lack of proliferation, with important variations in era occasions and confluencies (p < 0.05). MSC expressed the floor antigens CD90, CD44, and CD29, however CD73 and CD105 detection was low in all tradition media. Adipogenic and osteogenic differentiation led to related ends in MSC from totally different tradition media. The buffy coat methodology is beneficial to provide equine platelet focus with elevated platelet and decreased white blood cell content material in giant scales. The ePL obtained helps MSC enlargement related as FBS when used on the similar focus (10%). Additional investigations into equine MSC performance in tradition with ePL ought to observe.

Impact of Listeria monocytogenes on intestinal stem cells within the co-tradition mannequin of small intestinal organoids

 

Listeria monocytogenes is a foodborne pathogen that causes systemic infections by crossing the intestinal barrier. Nevertheless, in vitro evaluation of the interplay of L. monocytogenes and small intestinal epithelium has but to be totally elucidated. To review host responses from intestinal epithelium throughout L. monocytogenes an infection, we used the co-culture mannequin of small intestinal organoids and L. monocytogenes. Outcomes confirmed that L. monocytogenes mediated harm to intestinal epithelium, particularly intestinal stem cells. L. monocytogenes was discovered to cut back budding price and enhance mortality of organoids. Furthermore, it affected the proliferation of epithelial cells and numbers of secretory cells. As well as, it was demonstrated that L. monocytogenes stimulated a discount within the variety of Lgr5+ stem cells. Moreover, L. monocytogenes affected the expression of Hes1, Math1 and Sox9 to intrude with the differentiation of intestinal stem cells. Collectively, our findings reveal the results of L. monocytogenes an infection on intestinal stem cells and reveal that small intestinal organoid is an acceptable experimental mannequin for learning intestinal epithelium-pathogen interactions.

Paclitaxel is a widely known chemotherapeutic agent extensively utilized as a remedy for numerous kinds of cancers. In vitro tradition of Corylus avellana has been named as a promising and low-cost technique for paclitaxel manufacturing. Fungal elicitors have been reported as a formidable technique for enhancing paclitaxel biosynthesis in cell suspension tradition (CSC) of C. avellana. The targets of this analysis have been to forecast and optimize development and paclitaxel biosynthesis based mostly on 4 enter variables together with cell extract (CE) and tradition filtrate (CF) focus ranges, elicitor including day and CSC harvesting time in C. avellana cell tradition, as a case research, utilizing normal regression neural network-fruit fly optimization algorithm (GRNN-FOA) by way of knowledge mining strategy for the primary time.

Scalable Production of Equine Platelet Lysate for Multipotent Mesenchymal Stromal Cell Culture

Cell and tissue system able to automated tradition, stimulation, and monitor with the goal of suggestions management of organs-on-a-chip

This paper presents progress within the automation of cell and tissue techniques and makes an attempt towards the in situ suggestions management of organs-on-a-chip. Our research goals to realize suggestions management of a cell and tissue system by a private pc (PC), whereas most research on organs-on-a-chip concentrate on the automation of standing monitoring. The applied system consists of subsystems together with automated tradition, stimulation, and monitoring. The monitoring perform gives imaging in addition to sampling and shelling out together with an exterior analyzer. Particular person subsystems might be mixed accordingly. First, monitoring of skeletal muscle (SM) and adipose tissues utilizing this technique was demonstrated.

FINAL WASH SOLUTION/CULTURE MEDIUM

99-785-CV CORNING 500 mL/pk
EUR 164
Description: Specialty Media; Islet products

Human CD34+ Cell Culture Medium

P771 101Bio - Ask for price

Insect Cell Medium: TNM-FH Insect Culture Medium

ABP-MED-10001 Allele Biotech 1 liter Ask for price

Insect Cell Medium: Serum-Free Insect Culture Medium

ABP-MED-10002 Allele Biotech 1 liter Ask for price

CAMPY BLOOD FREE SELECTIVE MEDIUM

C03-102-10kg Alphabiosciences 10 kg Ask for price

CAMPY BLOOD FREE SELECTIVE MEDIUM

C03-102-2kg Alphabiosciences 2kg Ask for price

CAMPY BLOOD FREE SELECTIVE MEDIUM

C03-102-500g Alphabiosciences 500 g Ask for price

Anti-Blood Group A Culture supernatant

99-805-L001 ExBio 1.0 ml
EUR 122

Anti-Blood Group B Culture supernatant

99-806-L001 ExBio 1.0 ml
EUR 122

Anti-Blood Group A1B Culture supernatant

99-807-L001 ExBio 1.0 ml
EUR 122

Anti-Blood Group A Culture supernatant

99-809-L001 ExBio 1.0 ml
EUR 122

Anti-Blood Group ABH Culture supernatant

99-812-L001 ExBio 1.0 ml
EUR 122

CORNING® ENDOTHELIAL CELL CULTURE MEDIUM, KIT

355054 CORNING 1/pk
EUR 399
Description: Advanced Cells - DL; Adv Cell Misc Custom - DL

3D Cell Culture Gel, medium (Col-Tgel)

P720M-10 101Bio - Ask for price

3D Cell Culture Gel, medium (Col-Tgel)

P720M-2 101Bio - Ask for price

MIAMI MEDIUM #1A CULTURE MEDIUM (WITH HUMAN SERUM ALBUMIN, WITHOUT ANTIBIOTICS)

98-021-CV CORNING 500 mL/pk
EUR 124
Description: Specialty Media; Islet products

3D Cell Culture Gel 2ml x(soft+medium+hard)

P720SMH-6 101Bio - Ask for price

AXYPREP MAG TISSUE-BLOOD GDNA- MEDIUM - 384 PREPS

MAG-T-GDNA-M CORNING 1/pk
EUR 585
Description: Bioscience Mag Beads; Magnetic Tissue GDNA

Rat Lymph PrimaCell2: Normal Lymphatic Blood Vessel Endothelial Cells Growth Medium

9-25071 CHI Scientific 5 x 100 ml Ask for price

Mouse Lymph PrimaCell2: Normal Lymphatic Blood Vessel Endothelial Cells Growth Medium

9-32072 CHI Scientific 5 x 100 ml Ask for price

Human Lymph PrimaCell2: Normal Lymphatic Blood Vessel Endothelial Cells Growth Medium

9-46088 CHI Scientific 5 x 100 ml Ask for price

Monoclonal Anti-Dengue Virus Type 1-4 (pan, E antigen) IgG, culture medium

AB-21121 Alpha Diagnostics 0.5 ml
EUR 529

Cell Culture Grade Water, Cell Culture Tested, Sterile

CCM1081-500 Bio Basic 500 mL
EUR 61.24

Cell Culture Grade Water, Cell Culture Tested, Sterile

CCM2081-1000 Bio Basic 1000 mL
EUR 68.87

ExoStep Cell Culture

ExoS-25-C9 ImmunoStep 25 test
EUR 550.5

ExoStepTM Culture + Standard

ExoS-25-CST9 ImmunoStep 25 test
EUR 726

Mouse monoclonal Anti-Lymphocytic choriomeningitis virus (LCMV) Capsid Protein 1 (VP1) antibody, culture medium

LCMV15-M Alpha Diagnostics 1 ml
EUR 482

EC MEDIUM

E05-100-10kg Alphabiosciences 10 kg
EUR 814

EC MEDIUM

E05-100-2Kg Alphabiosciences 2 Kg
EUR 216

EC MEDIUM

E05-100-500g Alphabiosciences 500 g
EUR 95

COLIFORM MEDIUM

C03-127-10kg Alphabiosciences 10 kg
EUR 1324

COLIFORM MEDIUM

C03-127-2kg Alphabiosciences 2kg
EUR 327

COLIFORM MEDIUM

C03-127-500g Alphabiosciences 500 g
EUR 125

HLP MEDIUM

H08-107-10kg Alphabiosciences 10 kg
EUR 2278

HLP MEDIUM

H08-107-2Kg Alphabiosciences 2 Kg
EUR 534

HLP MEDIUM

H08-107-500g Alphabiosciences 500 g
EUR 182

VALI Medium

450 AthenaES 500 ml
EUR 265

DC MEDIUM

D04-117-10kg Alphabiosciences 10 kg
EUR 1579

DC MEDIUM

D04-117-2kg Alphabiosciences 2kg
EUR 382

DC MEDIUM

D04-117-500g Alphabiosciences 500 g
EUR 140

SIM MEDIUM

S19-110-10kg Alphabiosciences 10 kg
EUR 1021

SIM MEDIUM

S19-110-2kg Alphabiosciences 2kg
EUR 261

SIM MEDIUM

S19-110-500g Alphabiosciences 500 g
EUR 107

SOB MEDIUM

S19-124-10kg Alphabiosciences 10 kg
EUR 965

SOB MEDIUM

S19-124-2kg Alphabiosciences 2kg
EUR 249

SOB MEDIUM

S19-124-500g Alphabiosciences 500 g
EUR 104

Advanced Medium

C0003-04 Addexbio RT 500 mL Bottle
EUR 103

Medium 199

C0012-01 Addexbio RT 500 mL Bottle
EUR 105

NeuroProgenitor Medium

NM42400 Neuromics 125 ml
EUR 304

Heller's Medium

CP014-010 GenDepot 10X1L
EUR 99

Heller's Medium

CP014-500 GenDepot 50L
EUR 126

Hoagland's Medium

CP015-010 GenDepot 10X1L
EUR 99

Hoagland's Medium

CP015-500 GenDepot 50L
EUR 126

M9CA Medium

SD7025 Bio Basic 250g
EUR 71.75

YM Medium

SD7031 Bio Basic 250g
EUR 70.45

TYGPN Medium

SD7032 Bio Basic 250g
EUR 70.01

M63 Medium

SD7033 Bio Basic 250g
EUR 71.75

M9 Medium

SD7024 Bio Basic 250g
EUR 71.75

A Medium

DJ1018 Bio Basic 100g
EUR 84.8

Hepes Cell Culture Grade

AK3268-0250 Akron Biotech 250g Ask for price

Hepes Cell Culture Grade

AK3268-0500 Akron Biotech 500g Ask for price

Hepes Cell Culture Grade

AK3268-1000 Akron Biotech 1kg Ask for price

Apotransferrin tissue culture grade

30-AA15TC Fitzgerald 100 mg
EUR 127
Description: Apotransferrin, Human Plasma, Tissue Culture Grade

PetakaG3 FLAT Culture Devices

Petaka-FLAT Neuromics 300 Petaka Devices
EUR 10164

PetakaG3 HOT Culture Devices

Petaka-HOT Neuromics 300 Petaka Devices
EUR 10164

PetakaG3 LOT Culture Devices

Petaka-LOT Neuromics 300 Petaka Devices
EUR 10164

Water, Cell Culture Grade

CA018-600 GenDepot 6x1000ml
EUR 124

Ethanolamine, Cell Culture tested

CA071-010 GenDepot 100ml
EUR 97

Insect Cell Medium: Grace?s Insect Medium (Unsupplemented)

ABP-MED-10004 Allele Biotech 1 liter Ask for price

EC MEDIUM, MODIFIED

E05-108-10kg Alphabiosciences 10 kg
EUR 858

EC MEDIUM, MODIFIED

E05-108-2Kg Alphabiosciences 2 Kg
EUR 226

EC MEDIUM, MODIFIED

E05-108-500g Alphabiosciences 500 g
EUR 98

A-1 MEDIUM

A01-116-10kg Alphabiosciences 10 kg
EUR 1034

A-1 MEDIUM

A01-116-2Kg Alphabiosciences 2 Kg
EUR 264

A-1 MEDIUM

A01-116-500g Alphabiosciences 500 g
EUR 108

CARY & BLAIR MEDIUM

C03-131-10kg Alphabiosciences 10 kg
EUR 2185

CARY & BLAIR MEDIUM

C03-131-2Kg Alphabiosciences 2 Kg
EUR 514

CARY & BLAIR MEDIUM

C03-131-500g Alphabiosciences 500 g
EUR 176

FLUID THIOGLYCOLLATE MEDIUM

F06-101-10kg Alphabiosciences 10 kg
EUR 792

FLUID THIOGLYCOLLATE MEDIUM

F06-101-2kg Alphabiosciences 2kg
EUR 211

FLUID THIOGLYCOLLATE MEDIUM

F06-101-500g Alphabiosciences 500 g
EUR 94

TERRIFIC BROTH MEDIUM

T20-114-10kg Alphabiosciences 10 kg
EUR 675

TERRIFIC BROTH MEDIUM

T20-114-2kg Alphabiosciences 2kg
EUR 186

TERRIFIC BROTH MEDIUM

T20-114-500g Alphabiosciences 500 g
EUR 87

THIOGLYCOLLATE MEDIUM, BREWER

T20-118-10kg Alphabiosciences 10 kg
EUR 1364

THIOGLYCOLLATE MEDIUM, BREWER

T20-118-2kg Alphabiosciences 2kg
EUR 336

THIOGLYCOLLATE MEDIUM, BREWER

T20-118-500g Alphabiosciences 500 g
EUR 128

2XYT MICROBIAL MEDIUM

X24-102-10kg Alphabiosciences 10 kg
EUR 614

2XYT MICROBIAL MEDIUM

X24-102-2Kg Alphabiosciences 2 Kg
EUR 173

2XYT MICROBIAL MEDIUM

X24-102-500g Alphabiosciences 500 g
EUR 83

SuperMOUNT? Mounting Medium

1211-20 Biovision
EUR 201

OF BASAL MEDIUM

O15-100-10kg Alphabiosciences 10 kg Ask for price

OF BASAL MEDIUM

O15-100-2kg Alphabiosciences 2kg Ask for price

OF BASAL MEDIUM

O15-100-500g Alphabiosciences 500 g Ask for price

MOTILITY TEST MEDIUM

M13-121-10kg Alphabiosciences 10 kg
EUR 1528

MOTILITY TEST MEDIUM

M13-121-2Kg Alphabiosciences 2 Kg
EUR 371

MOTILITY TEST MEDIUM

M13-121-500g Alphabiosciences 500 g
EUR 137

REINFORCED CLOSTRIDIUM MEDIUM

R18-105-10kg Alphabiosciences 10 kg
EUR 1194

REINFORCED CLOSTRIDIUM MEDIUM

R18-105-2kg Alphabiosciences 2kg
EUR 299

REINFORCED CLOSTRIDIUM MEDIUM

R18-105-500g Alphabiosciences 500 g
EUR 118

Sepadextran-25 Medium

SD25M-100 ABTBeads 100 g
EUR 271

Sepadextran-25 Medium

SD25M-500 ABTBeads 500 g
EUR 1036

Sepadextran-50 Medium

SD50M-100 ABTBeads 100 g
EUR 344

Sepadextran-50 Medium

SD50M-500 ABTBeads 500 g
EUR 1285

Recovery Medium - 6x12mL

1711 Intact Genomics 4/PK
EUR 128

EverBrite Mounting Medium

23001 Biotium 10mL
EUR 178
Description: Minimum order quantity: 1 unit of 10mL

AddexBio Cryopreservation Medium

C0002-20mL Addexbio RT vial
EUR 104

Aqueous Mounting Medium

AR1018 BosterBio 10mL X5 (Enough for 800-1200 assays)
EUR 80

Antifade Mounting Medium

AR1109 BosterBio 10mL (for 1000 assays)
EUR 65

RPMI 1640 Medium

abx098879-500ml Abbexa 500 ml
EUR 175

Agarose, medium EEO

GE4750-100G Glentham Life Sciences 100 g
EUR 166

Agarose, medium EEO

GE4750-500G Glentham Life Sciences 500 g
EUR 429

Schwann Growth Medium

SGM001 Neuromics 500 ml
EUR 244

Hepatocyte Maintenance Medium

HM42600 Neuromics 100 ml
EUR 409

Neuron Growth Medium

HNM001 Neuromics 500 ml
EUR 331

MCDB 131 Medium

CM034-050 GenDepot 500ml
EUR 85

MCDB 131 Medium

CM034-300 GenDepot 6x500ml
EUR 165

MCDB 131 Medium

CM034-310 GenDepot 10x500ml
EUR 219

MCDB 131 Medium

CM034-320 GenDepot 20x500ml
EUR 340

MCDB 131 Medium

CM034-350 GenDepot 50x500ml
EUR 585

Williams' Medium E

CM063-050 GenDepot 500ml
EUR 91

Williams' Medium E

CM063-300 GenDepot 6x500ml
EUR 266

Williams' Medium E

CM063-310 GenDepot 10x500ml
EUR 313

Williams' Medium E

CM063-320 GenDepot 20x500ml
EUR 604

Williams' Medium E

CM063-350 GenDepot 50x500ml
EUR 975

Cereal Crops Medium

CP003-005 GenDepot 5L
EUR 113

Cereal Crops Medium

CP003-010 GenDepot 10X1L
EUR 169

Kao Michayluk Medium

CP017-010 GenDepot 10X1L
EUR 99

Kao Michayluk Medium

CP017-500 GenDepot 50L
EUR 126

Orchid Maintenance Medium

CP037-010 GenDepot 10X1L
EUR 109

Orchid Maintenance Medium

CP037-500 GenDepot 50L
EUR 138

Woody Plants Medium

CP040-010 GenDepot 10X1L
EUR 101

Woody Plants Medium

CP040-500 GenDepot 50L
EUR 126

H Medium Broth

DJ1019 Bio Basic 100g
EUR 60.88

FluoreGuard Mounting Medium

FMM030 ScyTek Laboratories 30 ml
EUR 96

FluoreGuard Mounting Medium

FMM060 ScyTek Laboratories 60 ml
EUR 116

FluoreGuard Mounting Medium

FMM500 ScyTek Laboratories 500 ml
EUR 327

FluoreGuard Mounting Medium

FMM999 ScyTek Laboratories 1000 ml
EUR 530

Blood PCR Kit

20-abx09801320ulSystems Abbexa
  • EUR 258.00
  • EUR 565.00
  • 100 rxns × 20 ul Systems
  • 500 rxns × 20 ul Systems

Blood RNA Kit

20-abx098092 Abbexa
  • EUR 105.00
  • EUR 342.00
  • 2 rxns
  • 50 rxns

EZ-LINE Cell culture Dishes Tissue culture treated, 60X15mm, 500 per case

SP31171 Bio Basic 1CS, 500UNIT
EUR 192.41

EZ-LINE Cell culture Dishes Tissue culture treated, 90x20mm, 200 per case

SP31212 Bio Basic 1CS, 200UNIT
EUR 135.51

ROCKER CELL CULTURE BAG, 20L

91-200-78 CORNING 1/pk
EUR 411
Description: Flexible Containers; Bioprocess Bags and Parts

ROCKER CELL CULTURE BAG, 10L

91-200-79 CORNING 1/pk
EUR 372
Description: Flexible Containers; Bioprocess Bags and Parts

ROCKER CELL CULTURE BAG, 2L

91-200-80 CORNING 1/pk
EUR 375
Description: Flexible Containers; Bioprocess Bags and Parts

ROCKER CELL CULTURE BAG, 50L

91-200-81 CORNING 1/pk
EUR 475
Description: Flexible Containers; Bioprocess Bags and Parts

CMRL 1066, CIT CULTURE MEDIA

98-304-CV CORNING 500 mL/pk
EUR 65
Description: Specialty Media; Islet products

Anti-Hu CD173 Culture supernatant

99-594-L001 ExBio 1.0 ml
EUR 122

6-well Cell Culture Plates

abx090688-50plates Abbexa 50 plates
EUR 476

6-well Cell Culture Plates

abx090688-6plates Abbexa 6 plates
EUR 119

Tissue Culture Plate, 12 Well

TCP20-12 Bio Basic 1 UNIT
EUR 53.48

Tissue Culture Plate, 48 Well

TCP20-48 Bio Basic 1 UNIT
EUR 53.48

Tissue Culture Plate, 6 Well

TCP20-6 Bio Basic 1 UNIT
EUR 53.48

Tissue Culture Plate, 96 Well

TCP20-96 Bio Basic 1 UNIT
EUR 53.48

Mouse ES Cell Culture Media

M16101 Neuromics 500 mL
EUR 1114

CardioDISC (Scaffold for cell culture)

C-01-01-EX Sceti 12 sheets Ask for price
Description: The CardioDISC (Scaffold for cell culture) is available in Europe and for worldwide shipping via Gentaur.

UltraPure Water, Cell Culture Tested

W9200-045 GenDepot 450ml
EUR 80

Rat Cartilage Growth Medium PrimaCell: Normal Articular Cartilage Growth Medium

9-25023 CHI Scientific 5 x 100 ml Ask for price

Human Cartilage Growth Medium PrimaCell: Normal Articular Cartilage Growth Medium

9-46043 CHI Scientific 5 x 100 ml Ask for price

Rat Lymph PrimaCell2: Normal Lymphatic Blood Vessel Endothelial Cells Growth Supplements with Serum (for 500 ml medium)

9-26071 CHI Scientific 1 Set Ask for price

Mouse Lymph PrimaCell2: Normal Lymphatic Blood Vessel Endothelial Cells Growth Supplements with Serum (for 500 ml medium)

9-33072 CHI Scientific 1 Set Ask for price

Human Lymph PrimaCell2: Normal Lymphatic Blood Vessel Endothelial Cells Growth Supplements with Serum (for 500 ml medium)

9-47088 CHI Scientific 1 Set Ask for price

EC MEDIUM WITH MUG

E05-101-10kg Alphabiosciences 10 kg
EUR 2351

EC MEDIUM WITH MUG

E05-101-2kg Alphabiosciences 2kg
EUR 550

EC MEDIUM WITH MUG

E05-101-500g Alphabiosciences 500 g
EUR 186

SILAC - Neural Basal Medium

428 AthenaES 500 ml
EUR 98

SILAC - SFM Insect Medium

429 AthenaES 500 ml
EUR 108
The spotlight of this paper is the appliance of the system to the suggestions management of the lipid droplet (LD) measurement, the place biochemical stimulation utilizing insulin and adrenaline is managed by a PC based on the obtained LD imaging knowledge. On this research, the system demonstrated its perform of sustaining the specified measurement of LDs. Our outcomes broaden the potential of PC-controllable cell and tissue techniques by addressing the problem of suggestions management of organs-on-a-chip. The PC-controllable cell and tissue techniques will contribute to residing systems-on-a-chip based mostly on homeostasis phenomena involving interactions between organs or tissues.

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  2. Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo
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