Co-culture of Human Stem Cell Derived Neurons and Oligodendrocyte Progenitor Cells

Co-culture of Human Stem Cell Derived Neurons and Oligodendrocyte Progenitor Cells

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Crosstalk between neurons and oligodendrocytes is necessary for correct mind functioning. A number of co-culture strategies have been developed to check oligodendrocyte maturation, myelination or the impact of oligodendrocytes on neurons. Nevertheless, most of those strategies include cells derived from animal fashions. Within the present protocol, we co-culture human neurons with human oligodendrocytes. Neurons and oligodendrocyte precursor cells (OPCs) have been differentiated individually from pluripotent stem cells in keeping with beforehand printed protocols. To review neuron-glia cross-talk, neurons and OPCs have been plated in co-culture mode in optimized situations for added 28 days, and ready for OPC maturation and neuronal morphology evaluation. To our information, this is without doubt one of the first neuron-OPC protocols containing all human cells. Particular neuronal abnormalities not noticed in mono-cultures of Tuberous Sclerosis Complicated (TSC) neurons, grew to become obvious when TSC neurons have been co-cultured with TSC OPCs. These outcomes present that this co-culture system can be utilized to check human neuron-OPC interactive mechanisms concerned in well being and illness.

Since their discovery, mesenchymal stromal cells (MSCs) have obtained lots of consideration, primarily resulting from their self-renewal potential and multilineage differentiation capability. For these causes, MSCs are a useful gizmo in cell biology and regenerative medication. On this article, we describe protocols to isolate MSCs from bone marrow (BM-MSCs) and adipose tissues (AT-MSCs), and strategies to tradition, characterize, and differentiate MSCs into osteoblasts, adipocytes, and chondrocytes. After the harvesting of cells from bone marrow by flushing the femoral diaphysis and enzymatic digestion of belly and inguinal adipose tissues, MSCs are chosen by their adherence to the plastic tissue tradition dish. Inside 7 days, MSCs attain 70% confluence and are prepared for use in subsequent experiments. The protocols described listed here are simple to carry out, cost-efficient, require minimal time, and yield a cell inhabitants wealthy in MSCs.

Technique for Main Epithelial Cell Tradition from the Rat Choroid Plexus

The choroid plexus consists of a community of secretory epithelial cells localized all through the lateral, third and fourth ventricles of the mind. Cerebrospinal fluid (CSF) is generated by the choroid plexus and launched into the ventricular setting. This biofluid incorporates an enriched supply of proteins, ions, and different signaling molecules for extracellular help of neurons and glial cells inside the central nervous system. On condition that different cells within the mind additionally launch elements into the CSF, in vitro investigations of choroid plexus operate are essential to isolate processes selectively occurring inside and launched from this tissue. Right here, we describe a protocol to isolate choroid plexus tissue from every of the ventricular areas, and the cell tradition situations required to help development and upkeep of those epithelial cells. This method permits for investigations of the purposeful significance of the choroid plexus, corresponding to for the examination of stimuli selling the discharge of development elements and extracellular vesicles (e.g., exosomes and microvesicles) from ventricle-specific choroid plexus epithelial cells.
Investigations into glial biology have contributed considerably in understanding the physiology and pathology of the nervous system. Nevertheless, intricacies of the neuron-glial and glial-glial interactions in vivo current important challenges whereas delineating the person cell-type contributions, thus making the in vitro methods exceedingly related to check glial biology. Nevertheless, acquiring optimum yield together with excessive purity has been difficult for microglial cultures. Right here we current a easy protocol to ascertain enriched astroglial in addition to microglial cultures from the neonatal rat spinal twine. This technique ends in extremely enriched astroglial and microglial cultures with maximal yield.

Quantification of Hepatitis B Virus Covalently Closed Round DNA in Contaminated Cell Tradition Fashions by Quantitative PCR

 

Persistence of the human hepatitis B virus (HBV) requires the upkeep of covalently closed round (ccc)DNA, the episomal genome reservoir in nuclei of contaminated hepatocytes. cccDNA elimination is a significant purpose in future healing therapies at present beneath growth. In cell tradition based mostly in vitro research, each hybridization- and amplification-based assays are at present used for cccDNA quantification. Southern blot, the present gold normal, is time-consuming and never sensible for a lot of samples. PCR-based strategies present restricted specificity when extreme HBV replicative intermediates are current. Now we have lately developed a real-time quantitative PCR protocol, by which whole mobile DNA plus all types of viral DNA are extracted by silica column. Subsequent incubation with T5 exonuclease effectively removes mobile DNA and all non-cccDNA types of viral DNA whereas cccDNA stays intact and may reliably be quantified by PCR. This technique has been used for measuring kinetics of cccDNA accumulation in a number of in vitro an infection fashions and the impact of antivirals on cccDNA. It allowed detection of cccDNA in non-human cells (major macaque and swine hepatocytes, and so forth.) reconstituted with the HBV receptor, human sodium taurocholate cotransporting polypeptide (NTCP). Right here we current an in depth protocol of this technique, together with a piece flowchart, schematic diagram and illustrations on learn how to calculate “cccDNA copies per (contaminated) cell”.

Human Umbilical Cord PrimaCell1: Normal Umbilical Artery Endothelial Cells Growth Medium
9-46106 5 x 100 ml Ask for price

Human Umbilical Cord PrimaCell4: Normal Umbilical Vein Endothelial Cells Growth Medium
9-46109 5 x 100 ml Ask for price

Human Peripheral Blood CD3+ T Cells, Cryopreserved
PBCD3-C10M - Ask for price

Human Peripheral Blood CD3+ T Cells, Cryopreserved
PBCD3-C20M - Ask for price

Human Peripheral Blood CD3+ T Cells, Fresh
PBCD3-F20M - Ask for price

Human Cord Blood CD34+ Cells Derived Endothelial Cells
HEC23 500,000+ Cells
EUR 1080

Human Umbilical Cord PrimaCell2: Normal Umbilical Artery Smooth Muscle Cells Growth Medium
9-46107 5 x 100 ml Ask for price

Human Umbilical Cord PrimaCell5: Normal Umbilical Vein Smooth Muscle Cells Growth Medium
9-46110 5 x 100 ml Ask for price

Human Umbilical Cord Tissue Preparation Buffer 1: Normal Umbilical Artery Endothelial Cells
9-80106 1 x 100 ml Ask for price

Human Umbilical Cord Tissue Preparation Buffer 4: Normal Umbilical Vein Endothelial Cells
9-80109 1 x 100 ml Ask for price

Human Umbilical Cord Tissue Preparation Buffer 2: Normal Umbilical Artery Smooth Muscle Cells
9-80107 1 x 100 ml Ask for price

Human Umbilical Cord Tissue Preparation Buffer 5: Normal Umbilical Vein Smooth Muscle Cells
9-80110 1 x 100 ml Ask for price

Rat Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells Growth Medium
9-25087 5 x 100 ml Ask for price

Mouse Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells Growth Medium
9-32091 5 x 100 ml Ask for price

Mouse Umbilical Cord Tissue Preparation Buffer: Normal Umbilical Vein Smooth Muscle Cells
9-80205 1 x 100 ml Ask for price

Rat Umbilical Cord Tissue Preparation Buffer: Normal Umbilical Vein Smooth Muscle Cells
9-80294 1 x 100 ml Ask for price

Human CD3+ T Cells (Pan T Cells), Cryopreserved
M1299-10
EUR 566

Human CD3+ T Cells (Pan T Cells), Cryopreserved
M1299-20
EUR 805

Human CD3+ T Cells (Pan T Cells), Cryopreserved
M1299-30
EUR 1327

Human CD3+ T Cells (Pan T Cells), Cryopreserved
M1299-40
EUR 1953

Human Cord Blood CD34+ Cells, Cryopreserved, single donor
CBCD34-C0.5M - Ask for price

Human Cord Blood CD34+ Cells, Cryopreserved, single donor 
CBCD34-C1M - Ask for price

Human Cord Blood CD34+ Cells, Cryopreserved, mixed donor 
CBCD34mix-C0.5M - Ask for price

Human Cord Blood CD34+ Cells, Cryopreserved, mixed donor 
CBCD34mix-C1M - Ask for price

Human Cord Blood CD34+ Cells, Cryopreserved, mixed donor 
CBCD34mix-C5M - Ask for price

Human Umbilical Cord OptiTDS1: Tissue Dissociation System
4-28106 1 Kit Ask for price

Human Umbilical Cord OptiTDS2: Tissue Dissociation System
4-28107 1 Kit Ask for price

Human Umbilical Cord OptiTDS4: Tissue Dissociation System
4-28109 1 Kit Ask for price

Human Umbilical Cord OptiTDS5: Tissue Dissociation System
4-28110 1 Kit Ask for price

Human Umbilical Cord PrimaCell1: Normal Umbilical Artery Endothelial Cells Growth Supplements with Serum (for 500 ml medium)
9-47106 1 Set Ask for price

Human Umbilical Cord PrimaCell4: Normal Umbilical Vein Endothelial Cells Growth Supplements with Serum (for 500 ml medium)
9-47109 1 Set Ask for price

Human ; Umbilical Vein Endothelial Cells
02-701 100ng
EUR 401
Description: The Human ; Umbilical Vein Endothelial Cells is available in Europe and for worldwide shipping via Gentaur.

Human Umbilical Vein Endothelial Cells
HEC01 500,000+ Cells frozen
EUR 440

Mouse Umbilical Cord OptiTDS: Tissue Dissociation System
4-28205 1 Kit Ask for price

Rat Umbilical Cord OptiTDS: Tissue Dissociation System
4-28294 1 Kit Ask for price

Human Umbilical Cord PrimaCell2: Normal Umbilical Artery Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)
9-47107 1 Set Ask for price

Human Umbilical Cord PrimaCell5: Normal Umbilical Vein Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)
9-47110 1 Set Ask for price

Rat Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)
9-26087 1 Set Ask for price

Mouse Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)
9-33091 1 Set Ask for price

Cancer Antigen AFP Antigen (Human Cord Blood)
VAng-Wyb8624-inquire inquire Ask for price
Description: Alpha Fetoprotein (66 kDa), Cancer antigen from Human Cord Blood, 1.73 mg/mL.

Human  Cord  Blood  CD34+  Cells (Mixed  Donors)
CBCD34M-05 - Ask for price

Human  Cord  Blood  CD34+  Cells (Mixed  Donors)
CBCD34M-1 - Ask for price

Human  Cord  Blood  CD34+  Cells (Mixed  Donors)
CBCD34M-5 - Ask for price

Immortalized Human Umbilical Vein Endothelial Cells - SV40
T0014 1x106 cells / 1.0 ml Ask for price

GFP Expressing Human Umbilical Vein Endothelial Cells
GF01 500,000+ Cells frozen
EUR 1040

Human Normal Peripheral Blood CD4+/CD25+ Regulatory T Cells (T reg), Cryopreserved
PBCD4-25-C2M - Ask for price

Human Normal Peripheral Blood CD4+/CD25+ Regulatory T Cells (T reg), Fresh
PBCD4-25-F2M - Ask for price

Human Umbilical Cord FibrOut1: Fibroblast Inhibitory System (for 500 ml medium)
7-15095 1 Kit Ask for price

Human Umbilical Cord FibrOut2: Fibroblast Inhibitory System (for 500 ml medium)
7-15096 1 Kit Ask for price

Human Umbilical Cord FibrOut4: Fibroblast Inhibitory System (for 500 ml medium)
7-15098 1 Kit Ask for price

Human Umbilical Cord FibrOut5: Fibroblast Inhibitory System (for 500 ml medium)
7-15099 1 Kit Ask for price

human umbilical vein endothelial cells, Peptide Aptamer, Biotinylated
AP-316-B 1 mg Ask for price

human umbilical vein endothelial cells, Peptide Aptamer, unlabeled
AP-316-U 5 mg Ask for price

Bovine Red Blood Cells
88R-B002 20 ml
EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Bovine Red Blood Cells

Chicken Red Blood Cells
88R-C002 20 ml
EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Chicken Red Blood Cells

Dog Red Blood Cells
88R-D004 10 ml
EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Dog Red Blood Cells

Goat Red Blood Cells
88R-G003 20 ml
EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Goat Red Blood Cells

Hamster Red Blood Cells
88R-H001 5 ml
EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Hamster Red Blood Cells

Horse Red Blood Cells
88R-H002 20 ml
EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Horse Red Blood Cells

Hamster Red Blood Cells
88R-H003 10 ml
EUR 489
Description: Washed and freshly prepared 10% suspension of Hamster Red Blood Cells

Mouse Red Blood Cells
88R-M001 10 ml
EUR 295
Description: Washed and freshly prepared 10% suspension of Mouse Red Blood Cells

Monkey Red Blood Cells
88R-M003 5 x 2 ml
EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Monkey Red Blood Cells

Mouse Red Blood Cells
88R-M004 5 x 2 ml
EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Mouse Red Blood Cells

Pig Red Blood Cells
88R-P002 20 ml
EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Pig Red Blood Cells

Rabbit Red Blood Cells
88R-R001 20 ml
EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Rabbit Red Blood Cells

Rat Red Blood Cells
88R-R002 5 x 2.0ml
EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Rat Red Blood Cells

Rat Red Blood Cells
88R-R005 25 ml
EUR 327
Description: Washed and freshly prepared 10% suspension of Rat Red Blood Cells

Sheep Red Blood Cells
88R-S001 100 ml
EUR 512
Description: Washed and freshly prepared 10% suspension of Sheep Red Blood Cells

Sheep Red Blood Cells
88R-S003 20 ml
EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Sheep Red Blood Cells

Sheep Red Blood Cells
88R-S004 10 ml
EUR 521
Description: Glutaraldehyde-stabilized freshly prepared albumin sensitised Sheep Red Blood Cells

Sheep Red Blood Cells
88R-S005 10 ml
EUR 446
Description: Glutaraldehyde-stabilized freshly prepared tanned Sheep Red Blood Cells

Turkey Red Blood Cells
88R-T001 20 ml
EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Turkey Red Blood Cells

Sheep Red Blood Cells
20R-RS001 10 ml
EUR 521
Description: Human IgG Senstitized Gluteraldehyde Stabilized Sheep Red Bllood Cells

Unprocessed Human Cord Blood from IRB-approved donors, Fresh
FC-001-F10 - Ask for price

Unprocessed Human Cord Blood from IRB-approved donors, Fresh
FC-001-F100 - Ask for price

Unprocessed Human Cord Blood from IRB-approved donors, Fresh
FC-001-F50 - Ask for price

Unprocessed Human Cord Blood from IRB-approved donors, Fresh
FC-001-F80 - Ask for price

Mouse Umbilical Cord FibrOut: Fibroblast Inhibitory System (for 500 ml medium)
7-15184 1 Kit Ask for price

Rat Umbilical Cord FibrOut: Fibroblast Inhibitory System (for 500 ml medium)
7-15264 1 Kit Ask for price

human umbilical vein endothelial cells, Peptide Aptamer, FITC labelled
AP-316-F 1 mg Ask for price

anti-CD3 T-cell
501-A-01mg 0,1 mg
EUR 267.5
Description: anti-CD3 T-cell

anti-CD3 T-cell
501-A-1000ug 1000 ug
EUR 1282.5
Description: anti-CD3 T-cell

CD3, T-Cell; Polyclonal
A00030 6 ml
EUR 213

CD3, T-Cell; Polyclonal
A00030-0100 100 ml
EUR 2250

CD3, T-Cell; Polyclonal
A00030.0025 25 ml
EUR 621

CD3, T-Cell; Polyclonal
A20030 2 ml
EUR 130

CORNING®HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS, HUVEC-2 CELLS (>5X105 CELLS), 1 CRYOVIAL/PACK
354151 1/pk
EUR 388
Description: Permeable Support - DL; Coated Transwells - DL

Guinea Pig Red Blood Cells
88R-1047 15 ml
EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Guinea Pig Red Blood Cells

Guinea Pig Red Blood Cells
88R-G002 25 ml
EUR 365
Description: Washed and freshly prepared 10% suspension of Guinea Pig Red Blood Cells

Guinea Pig Red Blood Cells
88R-P001 15 ml
EUR 273
Description: Glutaraldehyde-stabilized freshly prepared Guinea Pig Red Blood Cells

Rabbit Red Blood Cells (100%)
20-abx800015
  • EUR 648.00
  • EUR 509.00
  • 100 ml
  • 50 ml

Sheep Red Blood Cells (100%)
20-abx800032
  • EUR 356.00
  • EUR 314.00
  • 100 ml
  • 50 ml

Goat Red Blood Cells (100%)
20-abx800047
  • EUR 384.00
  • EUR 328.00
  • 100 ml
  • 50 ml

Horse Red Blood Cells (100%)
20-abx800062
  • EUR 453.00
  • EUR 551.00
  • EUR 384.00
  • 100 ml
  • 500 ml
  • 50 ml

Horse Red Blood Cells (100%)
abx800062-1L 1 L
EUR 746

Donkey Red Blood Cells (100%)
20-abx800077
  • EUR 676.00
  • EUR 843.00
  • EUR 481.00
  • 100 ml
  • 500 ml
  • 50 ml

Donkey Red Blood Cells (100%)
abx800077-1L 1 L
EUR 1191

Camel Red Blood Cells (100%)
20-abx800092
  • EUR 746.00
  • EUR 1636.00
  • EUR 578.00
  • 100 ml
  • 500 ml
  • 50 ml

Camel Red Blood Cells (100%)
abx800092-1L 1 L
EUR 3042

Dog Red Blood Cells (100%)
20-abx800122
  • EUR 592.00
  • EUR 481.00
  • 100 ml
  • 50 ml

Chicken Red Blood Cells (100%)
20-abx800137
  • EUR 384.00
  • EUR 328.00
  • 100 ml
  • 50 ml

VEGF-stimulated human umbilical vein endothelial cells, Peptide Aptamer, Biotinylated
AP-339-B 1 mg Ask for price

VEGF-stimulated human umbilical vein endothelial cells, Peptide Aptamer, unlabeled
AP-339-U 5 mg Ask for price

Neural Stem Cells (iPSC from Blood Cells; Male)
ASE-9234 1 vial (1 x 10^6)
EUR 414.5
Description: 12 month

Neural Stem Cells (iPSC from Blood Cells; Female)
ASE-9234F 1 vial (1 x 10^6)
EUR 414.5
Description: 12 month

VEGF-stimulated human umbilical vein endothelial cells, Peptide Aptamer, FITC labelled
AP-339-F 1 mg Ask for price

Beagle dog Red Blood Cells (100%)
20-abx800107
  • EUR 829.00
  • EUR 662.00
  • 100 ml
  • 50 ml

Rabbit RED BLOOD CELLS HEPARIN 500mL*
31159-5 500mL
EUR 821.9

Neurons (iPSC from Blood Cells; Male)
ASE-9321 1 vial (1 x 10^6)
EUR 313.25
Description: 12 month

Neurons (iPSC from Blood Cells; Female)
ASE-9321F 1 vial (1 x 10^6)
EUR 313.25
Description: 12 month

anti-CD45 RA B-cells, T-cells, NK-cells
516-A-01mg 0,1 mg
EUR 267.5
Description: anti-CD45 RA B-cells, T-cells, NK-cells

anti-CD45 RA B-cells, T-cells, NK-cells
516-A-1000ug 1000 ug
EUR 1282.5
Description: anti-CD45 RA B-cells, T-cells, NK-cells

Spinal Cord
PC35106 1 Spinal cord
EUR 396

Spinal Cord
PC35146 Spinal Cord, >200,000 Cells
EUR 396

Spinal Cord
PC37110 5 Million Cells
EUR 800

Human Normal Peripheral Blood Mononuclear Cells (PBMC), Fresh
BMC-F500M - Ask for price

Human Normal Peripheral Blood Mononuclear Cells (PBMC), Cryopreserved
PBMC-C100M - Ask for price

Human Normal Peripheral Blood Mononuclear Cells (PBMC), Cryopreserved
PBMC-C10M - Ask for price

Human Normal Peripheral Blood Mononuclear Cells (PBMC), Cryopreserved
PBMC-C20M - Ask for price

Human Normal Peripheral Blood Mononuclear Cells (PBMC), Cryopreserved
PBMC-C50M - Ask for price

Human Normal Peripheral Blood Mononuclear Cells (PBMC), Fresh
PBMC-F100M - Ask for price

Human Normal Peripheral Blood Mononuclear Cells (PBMC), Fresh
PBMC-F1B - Ask for price

Human Normal Peripheral Blood Mononuclear Cells (PBMC), Fresh
PBMC-F2B - Ask for price

Human Normal Peripheral Blood Mononuclear Cells (PBMC), Fresh
PBMC-F300M - Ask for price

Human  Cord  Blood  CD34+  Cells (Single  Donor)
CBCD34S-05 - Ask for price

Human  Cord  Blood  CD34+  Cells (Single  Donor)
CBCD34S-1 - Ask for price

Astrocytes Mature (iPSC from Blood Cells; Male)
ASE-9322M 1 vial (1 x 10^6)
EUR 455
Description: 12 month

Astrocytes Mature (iPSC from Blood Cells; Female)
ASE-9322MF 1 vial (1 x 10^6)
EUR 455
Description: 12 month

Astrocytes Precursors (iPSC from Blood Cells; Male)
ASE-9322P 1 vial (1 x 10^6)
EUR 374
Description: 12 month

Astrocytes Precursors (iPSC from Blood Cells; Female)
ASE-9322PF 1 vial (1 x 10^6)
EUR 374
Description: 12 month

Dopaminergic Neurons (iPSC from Blood Cells; Male)
ASE-9323 1 vial
EUR 374
Description: 12 month

Dopaminergic Neurons (iPSC from Blood Cells; Female)
ASE-9323F 1 vial
EUR 374
Description: 12 month

anti-CD4 T helper cells
502-A-01mg 0,1 mg
EUR 267.5
Description: anti-CD4 T helper cells

anti-CD4 T helper cells
502-A-1000ug 1000 ug
EUR 1282.5
Description: anti-CD4 T helper cells

anti-CD5 All T-cells
503-A-01mg 0,1 mg
EUR 267.5
Description: anti-CD5 All T-cells

anti-CD5 All T-cells
503-A-1000ug 1000 ug
EUR 1282.5
Description: anti-CD5 All T-cells
The germinal heart (GC) is the location the place B cells bear clonal growth, affinity-based choice, and differentiation into reminiscence B cells or plasma cells. It has been troublesome to elucidate regulatory mechanisms for the dynamic GC B cell maturation and differentiation, partly as a result of experimental manipulation of GC B cells in vivo has been restricted and no in vitro system has been out there that resembles B cell response in GC. Right here we describe the protocol for a tradition system named “induced GC B (iGB) tradition system” which may induce large growth of B cells that exhibit GC B cell-like phenotype, and thus it mimics the GC response. This protocol will be helpful to elucidate the molecular mechanisms of GC B cell differentiation.

 

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