Crosstalk between neurons and oligodendrocytes is necessary for correct mind functioning. A number of co-culture strategies have been developed to check oligodendrocyte maturation, myelination or the impact of oligodendrocytes on neurons. Nevertheless, most of those strategies include cells derived from animal fashions. Within the present protocol, we co-culture human neurons with human oligodendrocytes. Neurons and oligodendrocyte precursor cells (OPCs) have been differentiated individually from pluripotent stem cells in keeping with beforehand printed protocols. To review neuron-glia cross-talk, neurons and OPCs have been plated in co-culture mode in optimized situations for added 28 days, and ready for OPC maturation and neuronal morphology evaluation. To our information, this is without doubt one of the first neuron-OPC protocols containing all human cells. Particular neuronal abnormalities not noticed in mono-cultures of Tuberous Sclerosis Complicated (TSC) neurons, grew to become obvious when TSC neurons have been co-cultured with TSC OPCs. These outcomes present that this co-culture system can be utilized to check human neuron-OPC interactive mechanisms concerned in well being and illness.
Since their discovery, mesenchymal stromal cells (MSCs) have obtained lots of consideration, primarily resulting from their self-renewal potential and multilineage differentiation capability. For these causes, MSCs are a useful gizmo in cell biology and regenerative medication. On this article, we describe protocols to isolate MSCs from bone marrow (BM-MSCs) and adipose tissues (AT-MSCs), and strategies to tradition, characterize, and differentiate MSCs into osteoblasts, adipocytes, and chondrocytes. After the harvesting of cells from bone marrow by flushing the femoral diaphysis and enzymatic digestion of belly and inguinal adipose tissues, MSCs are chosen by their adherence to the plastic tissue tradition dish. Inside 7 days, MSCs attain 70% confluence and are prepared for use in subsequent experiments. The protocols described listed here are simple to carry out, cost-efficient, require minimal time, and yield a cell inhabitants wealthy in MSCs.
Technique for Main Epithelial Cell Tradition from the Rat Choroid Plexus
The choroid plexus consists of a community of secretory epithelial cells localized all through the lateral, third and fourth ventricles of the mind. Cerebrospinal fluid (CSF) is generated by the choroid plexus and launched into the ventricular setting. This biofluid incorporates an enriched supply of proteins, ions, and different signaling molecules for extracellular help of neurons and glial cells inside the central nervous system. On condition that different cells within the mind additionally launch elements into the CSF, in vitro investigations of choroid plexus operate are essential to isolate processes selectively occurring inside and launched from this tissue. Right here, we describe a protocol to isolate choroid plexus tissue from every of the ventricular areas, and the cell tradition situations required to help development and upkeep of those epithelial cells. This method permits for investigations of the purposeful significance of the choroid plexus, corresponding to for the examination of stimuli selling the discharge of development elements and extracellular vesicles (e.g., exosomes and microvesicles) from ventricle-specific choroid plexus epithelial cells.
Investigations into glial biology have contributed considerably in understanding the physiology and pathology of the nervous system. Nevertheless, intricacies of the neuron-glial and glial-glial interactions in vivo current important challenges whereas delineating the person cell-type contributions, thus making the in vitro methods exceedingly related to check glial biology. Nevertheless, acquiring optimum yield together with excessive purity has been difficult for microglial cultures. Right here we current a easy protocol to ascertain enriched astroglial in addition to microglial cultures from the neonatal rat spinal twine. This technique ends in extremely enriched astroglial and microglial cultures with maximal yield.
Quantification of Hepatitis B Virus Covalently Closed Round DNA in Contaminated Cell Tradition Fashions by Quantitative PCR
Persistence of the human hepatitis B virus (HBV) requires the upkeep of covalently closed round (ccc)DNA, the episomal genome reservoir in nuclei of contaminated hepatocytes. cccDNA elimination is a significant purpose in future healing therapies at present beneath growth. In cell tradition based mostly in vitro research, each hybridization- and amplification-based assays are at present used for cccDNA quantification. Southern blot, the present gold normal, is time-consuming and never sensible for a lot of samples. PCR-based strategies present restricted specificity when extreme HBV replicative intermediates are current. Now we have lately developed a real-time quantitative PCR protocol, by which whole mobile DNA plus all types of viral DNA are extracted by silica column. Subsequent incubation with T5 exonuclease effectively removes mobile DNA and all non-cccDNA types of viral DNA whereas cccDNA stays intact and may reliably be quantified by PCR. This technique has been used for measuring kinetics of cccDNA accumulation in a number of in vitro an infection fashions and the impact of antivirals on cccDNA. It allowed detection of cccDNA in non-human cells (major macaque and swine hepatocytes, and so forth.) reconstituted with the HBV receptor, human sodium taurocholate cotransporting polypeptide (NTCP). Right here we current an in depth protocol of this technique, together with a piece flowchart, schematic diagram and illustrations on learn how to calculate “cccDNA copies per (contaminated) cell”.
Human Umbilical Cord PrimaCell1: Normal Umbilical Artery Endothelial Cells Growth Medium |
9-46106 |
CHI Scientific |
5 x 100 ml |
Ask for price |
Human Umbilical Cord PrimaCell4: Normal Umbilical Vein Endothelial Cells Growth Medium |
9-46109 |
CHI Scientific |
5 x 100 ml |
Ask for price |
Human Peripheral Blood CD3+Â T Cells, Cryopreserved |
PBCD3-C10M |
101Bio |
- |
Ask for price |
Human Peripheral Blood CD3+Â T Cells, Cryopreserved |
PBCD3-C20M |
101Bio |
- |
Ask for price |
Human Peripheral Blood CD3+Â T Cells, Fresh |
PBCD3-F20M |
101Bio |
- |
Ask for price |
Human Cord Blood CD34+ Cells Derived Endothelial Cells |
HEC23 |
Neuromics |
500,000+ Cells |
EUR 1080 |
Human Umbilical Cord PrimaCell2: Normal Umbilical Artery Smooth Muscle Cells Growth Medium |
9-46107 |
CHI Scientific |
5 x 100 ml |
Ask for price |
Human Umbilical Cord PrimaCell5: Normal Umbilical Vein Smooth Muscle Cells Growth Medium |
9-46110 |
CHI Scientific |
5 x 100 ml |
Ask for price |
Human Umbilical Cord Tissue Preparation Buffer 1: Normal Umbilical Artery Endothelial Cells |
9-80106 |
CHI Scientific |
1 x 100 ml |
Ask for price |
Human Umbilical Cord Tissue Preparation Buffer 4: Normal Umbilical Vein Endothelial Cells |
9-80109 |
CHI Scientific |
1 x 100 ml |
Ask for price |
Human Umbilical Cord Tissue Preparation Buffer 2: Normal Umbilical Artery Smooth Muscle Cells |
9-80107 |
CHI Scientific |
1 x 100 ml |
Ask for price |
Human Umbilical Cord Tissue Preparation Buffer 5: Normal Umbilical Vein Smooth Muscle Cells |
9-80110 |
CHI Scientific |
1 x 100 ml |
Ask for price |
Rat Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells Growth Medium |
9-25087 |
CHI Scientific |
5 x 100 ml |
Ask for price |
Mouse Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells Growth Medium |
9-32091 |
CHI Scientific |
5 x 100 ml |
Ask for price |
Mouse Umbilical Cord Tissue Preparation Buffer: Normal Umbilical Vein Smooth Muscle Cells |
9-80205 |
CHI Scientific |
1 x 100 ml |
Ask for price |
Rat Umbilical Cord Tissue Preparation Buffer: Normal Umbilical Vein Smooth Muscle Cells |
9-80294 |
CHI Scientific |
1 x 100 ml |
Ask for price |
Human CD3+ T Cells (Pan T Cells), Cryopreserved |
M1299-10 |
Biovision |
|
EUR 566 |
Human CD3+ T Cells (Pan T Cells), Cryopreserved |
M1299-20 |
Biovision |
|
EUR 805 |
Human CD3+ T Cells (Pan T Cells), Cryopreserved |
M1299-30 |
Biovision |
|
EUR 1327 |
Human CD3+ T Cells (Pan T Cells), Cryopreserved |
M1299-40 |
Biovision |
|
EUR 1953 |
Human Cord Blood CD34+ Cells, Cryopreserved, single donor |
CBCD34-C0.5M |
101Bio |
- |
Ask for price |
Human Cord Blood CD34+ Cells, Cryopreserved, single donor |
CBCD34-C1M |
101Bio |
- |
Ask for price |
Human Cord Blood CD34+ Cells, Cryopreserved, mixed donor |
CBCD34mix-C0.5M |
101Bio |
- |
Ask for price |
Human Cord Blood CD34+ Cells, Cryopreserved, mixed donor |
CBCD34mix-C1M |
101Bio |
- |
Ask for price |
Human Cord Blood CD34+ Cells, Cryopreserved, mixed donor |
CBCD34mix-C5M |
101Bio |
- |
Ask for price |
Human Umbilical Cord OptiTDS1: Tissue Dissociation System |
4-28106 |
CHI Scientific |
1 Kit |
Ask for price |
Human Umbilical Cord OptiTDS2: Tissue Dissociation System |
4-28107 |
CHI Scientific |
1 Kit |
Ask for price |
Human Umbilical Cord OptiTDS4: Tissue Dissociation System |
4-28109 |
CHI Scientific |
1 Kit |
Ask for price |
Human Umbilical Cord OptiTDS5: Tissue Dissociation System |
4-28110 |
CHI Scientific |
1 Kit |
Ask for price |
Human Umbilical Cord PrimaCell1: Normal Umbilical Artery Endothelial Cells Growth Supplements with Serum (for 500 ml medium) |
9-47106 |
CHI Scientific |
1 Set |
Ask for price |
Human Umbilical Cord PrimaCell4: Normal Umbilical Vein Endothelial Cells Growth Supplements with Serum (for 500 ml medium) |
9-47109 |
CHI Scientific |
1 Set |
Ask for price |
Human ; Umbilical Vein Endothelial Cells |
02-701 |
Sceti |
100ng |
EUR 401 |
Description: The Human ; Umbilical Vein Endothelial Cells is available in Europe and for worldwide shipping via Gentaur. |
Human Umbilical Vein Endothelial Cells |
HEC01 |
Neuromics |
500,000+ Cells frozen |
EUR 440 |
Mouse Umbilical Cord OptiTDS: Tissue Dissociation System |
4-28205 |
CHI Scientific |
1 Kit |
Ask for price |
Rat Umbilical Cord OptiTDS: Tissue Dissociation System |
4-28294 |
CHI Scientific |
1 Kit |
Ask for price |
Human Umbilical Cord PrimaCell2: Normal Umbilical Artery Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium) |
9-47107 |
CHI Scientific |
1 Set |
Ask for price |
Human Umbilical Cord PrimaCell5: Normal Umbilical Vein Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium) |
9-47110 |
CHI Scientific |
1 Set |
Ask for price |
Rat Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium) |
9-26087 |
CHI Scientific |
1 Set |
Ask for price |
Mouse Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium) |
9-33091 |
CHI Scientific |
1 Set |
Ask for price |
Cancer Antigen AFP Antigen (Human Cord Blood) |
VAng-Wyb8624-inquire |
Creative Biolabs |
inquire |
Ask for price |
Description: Alpha Fetoprotein (66 kDa), Cancer antigen from Human Cord Blood, 1.73 mg/mL. |
Human  Cord  Blood  CD34+  Cells (Mixed  Donors) |
CBCD34M-05 |
101Bio |
- |
Ask for price |
Human  Cord  Blood  CD34+  Cells (Mixed  Donors) |
CBCD34M-1 |
101Bio |
- |
Ask for price |
Human  Cord  Blood  CD34+  Cells (Mixed  Donors) |
CBCD34M-5 |
101Bio |
- |
Ask for price |
Immortalized Human Umbilical Vein Endothelial Cells - SV40 |
T0014 |
ABM |
1x106 cells / 1.0 ml |
Ask for price |
GFP Expressing Human Umbilical Vein Endothelial Cells |
GF01 |
Neuromics |
500,000+ Cells frozen |
EUR 1040 |
Human Normal Peripheral Blood CD4+/CD25+ Regulatory T Cells (T reg), Cryopreserved |
PBCD4-25-C2M |
101Bio |
- |
Ask for price |
Human Normal Peripheral Blood CD4+/CD25+ Regulatory T Cells (T reg), Fresh |
PBCD4-25-F2M |
101Bio |
- |
Ask for price |
Human Umbilical Cord FibrOut1: Fibroblast Inhibitory System (for 500 ml medium) |
7-15095 |
CHI Scientific |
1 Kit |
Ask for price |
Human Umbilical Cord FibrOut2: Fibroblast Inhibitory System (for 500 ml medium) |
7-15096 |
CHI Scientific |
1 Kit |
Ask for price |
Human Umbilical Cord FibrOut4: Fibroblast Inhibitory System (for 500 ml medium) |
7-15098 |
CHI Scientific |
1 Kit |
Ask for price |
Human Umbilical Cord FibrOut5: Fibroblast Inhibitory System (for 500 ml medium) |
7-15099 |
CHI Scientific |
1 Kit |
Ask for price |
human umbilical vein endothelial cells, Peptide Aptamer, Biotinylated |
AP-316-B |
Alpha Diagnostics |
1 mg |
Ask for price |
human umbilical vein endothelial cells, Peptide Aptamer, unlabeled |
AP-316-U |
Alpha Diagnostics |
5 mg |
Ask for price |
Bovine Red Blood Cells |
88R-B002 |
Fitzgerald |
20 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Bovine Red Blood Cells |
Chicken Red Blood Cells |
88R-C002 |
Fitzgerald |
20 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Chicken Red Blood Cells |
Dog Red Blood Cells |
88R-D004 |
Fitzgerald |
10 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Dog Red Blood Cells |
Goat Red Blood Cells |
88R-G003 |
Fitzgerald |
20 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Goat Red Blood Cells |
Hamster Red Blood Cells |
88R-H001 |
Fitzgerald |
5 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Hamster Red Blood Cells |
Horse Red Blood Cells |
88R-H002 |
Fitzgerald |
20 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Horse Red Blood Cells |
Hamster Red Blood Cells |
88R-H003 |
Fitzgerald |
10 ml |
EUR 489 |
Description: Washed and freshly prepared 10% suspension of Hamster Red Blood Cells |
Mouse Red Blood Cells |
88R-M001 |
Fitzgerald |
10 ml |
EUR 295 |
Description: Washed and freshly prepared 10% suspension of Mouse Red Blood Cells |
Monkey Red Blood Cells |
88R-M003 |
Fitzgerald |
5 x 2 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Monkey Red Blood Cells |
Mouse Red Blood Cells |
88R-M004 |
Fitzgerald |
5 x 2 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Mouse Red Blood Cells |
Pig Red Blood Cells |
88R-P002 |
Fitzgerald |
20 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Pig Red Blood Cells |
Rabbit Red Blood Cells |
88R-R001 |
Fitzgerald |
20 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Rabbit Red Blood Cells |
Rat Red Blood Cells |
88R-R002 |
Fitzgerald |
5 x 2.0ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Rat Red Blood Cells |
Rat Red Blood Cells |
88R-R005 |
Fitzgerald |
25 ml |
EUR 327 |
Description: Washed and freshly prepared 10% suspension of Rat Red Blood Cells |
Sheep Red Blood Cells |
88R-S001 |
Fitzgerald |
100 ml |
EUR 512 |
Description: Washed and freshly prepared 10% suspension of Sheep Red Blood Cells |
Sheep Red Blood Cells |
88R-S003 |
Fitzgerald |
20 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Sheep Red Blood Cells |
Sheep Red Blood Cells |
88R-S004 |
Fitzgerald |
10 ml |
EUR 521 |
Description: Glutaraldehyde-stabilized freshly prepared albumin sensitised Sheep Red Blood Cells |
Sheep Red Blood Cells |
88R-S005 |
Fitzgerald |
10 ml |
EUR 446 |
Description: Glutaraldehyde-stabilized freshly prepared tanned Sheep Red Blood Cells |
Turkey Red Blood Cells |
88R-T001 |
Fitzgerald |
20 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Turkey Red Blood Cells |
Sheep Red Blood Cells |
20R-RS001 |
Fitzgerald |
10 ml |
EUR 521 |
Description: Human IgG Senstitized Gluteraldehyde Stabilized Sheep Red Bllood Cells |
Unprocessed Human Cord Blood from IRB-approved donors, Fresh |
FC-001-F10 |
101Bio |
- |
Ask for price |
Unprocessed Human Cord Blood from IRB-approved donors, Fresh |
FC-001-F100 |
101Bio |
- |
Ask for price |
Unprocessed Human Cord Blood from IRB-approved donors, Fresh |
FC-001-F50 |
101Bio |
- |
Ask for price |
Unprocessed Human Cord Blood from IRB-approved donors, Fresh |
FC-001-F80 |
101Bio |
- |
Ask for price |
Mouse Umbilical Cord FibrOut: Fibroblast Inhibitory System (for 500 ml medium) |
7-15184 |
CHI Scientific |
1 Kit |
Ask for price |
Rat Umbilical Cord FibrOut: Fibroblast Inhibitory System (for 500 ml medium) |
7-15264 |
CHI Scientific |
1 Kit |
Ask for price |
human umbilical vein endothelial cells, Peptide Aptamer, FITC labelled |
AP-316-F |
Alpha Diagnostics |
1 mg |
Ask for price |
anti-CD3 T-cell |
501-A-01mg |
Virogen |
0,1 mg |
EUR 267.5 |
|
Description: anti-CD3 T-cell |
anti-CD3 T-cell |
501-A-1000ug |
Virogen |
1000 ug |
EUR 1282.5 |
|
Description: anti-CD3 T-cell |
CORNING®HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS, HUVEC-2 CELLS (>5X105 CELLS), 1 CRYOVIAL/PACK |
354151 |
CORNING |
1/pk |
EUR 388 |
Description: Permeable Support - DL; Coated Transwells - DL |
Guinea Pig Red Blood Cells |
88R-1047 |
Fitzgerald |
15 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Guinea Pig Red Blood Cells |
Guinea Pig Red Blood Cells |
88R-G002 |
Fitzgerald |
25 ml |
EUR 365 |
Description: Washed and freshly prepared 10% suspension of Guinea Pig Red Blood Cells |
Guinea Pig Red Blood Cells |
88R-P001 |
Fitzgerald |
15 ml |
EUR 273 |
Description: Glutaraldehyde-stabilized freshly prepared Guinea Pig Red Blood Cells |
Rabbit Red Blood Cells (100%) |
20-abx800015 |
Abbexa |
|
|
|
Sheep Red Blood Cells (100%) |
20-abx800032 |
Abbexa |
|
|
|
Goat Red Blood Cells (100%) |
20-abx800047 |
Abbexa |
|
|
|
Horse Red Blood Cells (100%) |
20-abx800062 |
Abbexa |
-
EUR 453.00
-
EUR 551.00
-
EUR 384.00
|
|
|
Horse Red Blood Cells (100%) |
abx800062-1L |
Abbexa |
1 L |
EUR 746 |
|
Donkey Red Blood Cells (100%) |
20-abx800077 |
Abbexa |
-
EUR 676.00
-
EUR 843.00
-
EUR 481.00
|
|
|
Donkey Red Blood Cells (100%) |
abx800077-1L |
Abbexa |
1 L |
EUR 1191 |
|
Camel Red Blood Cells (100%) |
20-abx800092 |
Abbexa |
-
EUR 746.00
-
EUR 1636.00
-
EUR 578.00
|
|
|
Camel Red Blood Cells (100%) |
abx800092-1L |
Abbexa |
1 L |
EUR 3042 |
|
Dog Red Blood Cells (100%) |
20-abx800122 |
Abbexa |
|
|
|
Chicken Red Blood Cells (100%) |
20-abx800137 |
Abbexa |
|
|
|
VEGF-stimulated human umbilical vein endothelial cells, Peptide Aptamer, Biotinylated |
AP-339-B |
Alpha Diagnostics |
1 mg |
Ask for price |
VEGF-stimulated human umbilical vein endothelial cells, Peptide Aptamer, unlabeled |
AP-339-U |
Alpha Diagnostics |
5 mg |
Ask for price |
Neural Stem Cells (iPSC from Blood Cells; Male) |
ASE-9234 |
Applied StemCell |
1 vial (1 x 10^6) |
EUR 414.5 |
Description: 12 month |
Neural Stem Cells (iPSC from Blood Cells; Female) |
ASE-9234F |
Applied StemCell |
1 vial (1 x 10^6) |
EUR 414.5 |
Description: 12 month |
VEGF-stimulated human umbilical vein endothelial cells, Peptide Aptamer, FITC labelled |
AP-339-F |
Alpha Diagnostics |
1 mg |
Ask for price |
Beagle dog Red Blood Cells (100%) |
20-abx800107 |
Abbexa |
|
|
|
Rabbit RED BLOOD CELLS HEPARIN 500mL* |
31159-5 |
Pel-Freez |
500mL |
EUR 821.9 |
Neurons (iPSC from Blood Cells; Male) |
ASE-9321 |
Applied StemCell |
1 vial (1 x 10^6) |
EUR 313.25 |
Description: 12 month |
Neurons (iPSC from Blood Cells; Female) |
ASE-9321F |
Applied StemCell |
1 vial (1 x 10^6) |
EUR 313.25 |
Description: 12 month |
anti-CD45 RA B-cells, T-cells, NK-cells |
516-A-01mg |
Virogen |
0,1 mg |
EUR 267.5 |
|
Description: anti-CD45 RA B-cells, T-cells, NK-cells |
anti-CD45 RA B-cells, T-cells, NK-cells |
516-A-1000ug |
Virogen |
1000 ug |
EUR 1282.5 |
|
Description: anti-CD45 RA B-cells, T-cells, NK-cells |
Spinal Cord |
PC35106 |
Neuromics |
1 Spinal cord |
EUR 396 |
Spinal Cord |
PC35146 |
Neuromics |
Spinal Cord, >200,000 Cells |
EUR 396 |
Spinal Cord |
PC37110 |
Neuromics |
5 Million Cells |
EUR 800 |
Human Normal Peripheral Blood Mononuclear Cells (PBMC), Fresh |
BMC-F500M |
101Bio |
- |
Ask for price |
Human Normal Peripheral Blood Mononuclear Cells (PBMC), Cryopreserved |
PBMC-C100M |
101Bio |
- |
Ask for price |
Human Normal Peripheral Blood Mononuclear Cells (PBMC), Cryopreserved |
PBMC-C10M |
101Bio |
- |
Ask for price |
Human Normal Peripheral Blood Mononuclear Cells (PBMC), Cryopreserved |
PBMC-C20M |
101Bio |
- |
Ask for price |
Human Normal Peripheral Blood Mononuclear Cells (PBMC), Cryopreserved |
PBMC-C50M |
101Bio |
- |
Ask for price |
Human Normal Peripheral Blood Mononuclear Cells (PBMC), Fresh |
PBMC-F100M |
101Bio |
- |
Ask for price |
Human Normal Peripheral Blood Mononuclear Cells (PBMC), Fresh |
PBMC-F1B |
101Bio |
- |
Ask for price |
Human Normal Peripheral Blood Mononuclear Cells (PBMC), Fresh |
PBMC-F2B |
101Bio |
- |
Ask for price |
Human Normal Peripheral Blood Mononuclear Cells (PBMC), Fresh |
PBMC-F300M |
101Bio |
- |
Ask for price |
Human  Cord  Blood  CD34+  Cells (Single  Donor) |
CBCD34S-05 |
101Bio |
- |
Ask for price |
Human  Cord  Blood  CD34+  Cells (Single  Donor) |
CBCD34S-1 |
101Bio |
- |
Ask for price |
Astrocytes Mature (iPSC from Blood Cells; Male) |
ASE-9322M |
Applied StemCell |
1 vial (1 x 10^6) |
EUR 455 |
Description: 12 month |
Astrocytes Mature (iPSC from Blood Cells; Female) |
ASE-9322MF |
Applied StemCell |
1 vial (1 x 10^6) |
EUR 455 |
Description: 12 month |
Astrocytes Precursors (iPSC from Blood Cells; Male) |
ASE-9322P |
Applied StemCell |
1 vial (1 x 10^6) |
EUR 374 |
Description: 12 month |
Astrocytes Precursors (iPSC from Blood Cells; Female) |
ASE-9322PF |
Applied StemCell |
1 vial (1 x 10^6) |
EUR 374 |
Description: 12 month |
Dopaminergic Neurons (iPSC from Blood Cells; Male) |
ASE-9323 |
Applied StemCell |
1 vial |
EUR 374 |
Description: 12 month |
Dopaminergic Neurons (iPSC from Blood Cells; Female) |
ASE-9323F |
Applied StemCell |
1 vial |
EUR 374 |
Description: 12 month |
anti-CD4 T helper cells |
502-A-01mg |
Virogen |
0,1 mg |
EUR 267.5 |
|
Description: anti-CD4 T helper cells |
anti-CD4 T helper cells |
502-A-1000ug |
Virogen |
1000 ug |
EUR 1282.5 |
|
Description: anti-CD4 T helper cells |
anti-CD5 All T-cells |
503-A-01mg |
Virogen |
0,1 mg |
EUR 267.5 |
|
Description: anti-CD5 All T-cells |
anti-CD5 All T-cells |
503-A-1000ug |
Virogen |
1000 ug |
EUR 1282.5 |
|
Description: anti-CD5 All T-cells |
The germinal heart (GC) is the location the place B cells bear clonal growth, affinity-based choice, and differentiation into reminiscence B cells or plasma cells. It has been troublesome to elucidate regulatory mechanisms for the dynamic GC B cell maturation and differentiation, partly as a result of experimental manipulation of GC B cells in vivo has been restricted and no in vitro system has been out there that resembles B cell response in GC. Right here we describe the protocol for a tradition system named “induced GC B (iGB) tradition system” which may induce large growth of B cells that exhibit GC B cell-like phenotype, and thus it mimics the GC response. This protocol will be helpful to elucidate the molecular mechanisms of GC B cell differentiation.